不同肠内营养联合生长激素对烫伤大鼠肠黏膜损伤的保护作用。

The protective effect of different enteral nutrition combined with growth hormone on intestinal mucosal damage of scalded rats.

机构信息

Department of Traumatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong, University of Science and Technology, Wuhan 430030, PR China.

出版信息

Burns. 2010 Dec;36(8):1283-8. doi: 10.1016/j.burns.2010.04.003. Epub 2010 May 23.

DOI:10.1016/j.burns.2010.04.003

PMID:20547004

Abstract

OBJECTIVE

To investigate the protective effect of standard enteral nutrition (EN) and enteral immunonutrition (EIN) combined with recombinant human growth hormone rhGH on intestinal mucosa damage in scalded rats.

METHODS

Rats subjected to 30% total body surface area (TBSA III°), and they were divided randomly into five groups referred to as EN, EIN, EN+rhGH, EIN+rhGH and control groups. After injury on days 1, 4, 7 and 10, venous blood and intestinal mucus were collected to monitor the level of serum endotoxin, tumor necrosis factor-α (TNF-α) and the concentration of intestinal mucus s-IgA. The number of IgA plasma cells in intestinal tissue and proliferating cell nuclear antigen (PCNA) in intestinal mucosa were detected using the method of immunohistochemistry.

RESULTS

After injury on days 4, 7 and 10, PCNA value of the intestinal mucosa, the number of IgA plasma cells in intestinal lamina and the concentration of intestinal mucus s-IgA in EIN and EN+rhGH groups are significantly increased compared with those in EN group (P<0.05 or P<0.01), but serum endotoxin and TNF-α levels are reduced than those in EN group. After injury on days 4, 7 and 10, PCNA values, propria IgA plasma cell number and s-IgA concentration in EIN+rhGH group have no significant difference compared with those in EIN group (P>0.05), while the serum endotoxin and TNF-α levels are lower than those in EIN group.

CONCLUSIONS

EN or EIN combined with rhGH has the additional effect on treatment to reduce intestinal mucosa damage of scalded rats and strengthen the protection of intestinal mucosa damage.

摘要

目的

探讨标准肠内营养（EN）和肠内免疫营养（EIN）联合重组人生长激素（rhGH）对烫伤大鼠肠黏膜损伤的保护作用。

方法

将大鼠背部 30%（TBSA Ⅲ°）皮肤烫伤，随机分为 5 组，即 EN 组、EIN 组、EN+rhGH 组、EIN+rhGH 组和对照组。伤后 1、4、7、10 天分别采集静脉血和肠黏液，检测血清内毒素、肿瘤坏死因子-α（TNF-α）水平和肠黏液 s-IgA 浓度，免疫组化法检测肠黏膜 IgA 浆细胞数量和增殖细胞核抗原（PCNA）。

结果

伤后 4、7、10 天，EIN 组和 EN+rhGH 组大鼠肠黏膜 PCNA 值、肠黏膜固有层 IgA 浆细胞数和肠黏液 s-IgA 浓度均显著高于 EN 组（P<0.05 或 P<0.01），血清内毒素和 TNF-α水平低于 EN 组；伤后 4、7、10 天，EIN+rhGH 组大鼠肠黏膜 PCNA 值、固有层 IgA 浆细胞数和 s-IgA 浓度与 EIN 组比较差异无统计学意义（P>0.05），但血清内毒素和 TNF-α水平低于 EIN 组。