Cai Chen, Guo Guang-hua, Xu Qing-lian, Tang Yi-zhong, Liu Sheng
Department of Burns, First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2006 Oct;18(10):609-12.
To investigate the protective effects of enteral immuno-nutrition on intestinal mucosa injury following severe burn.
Sixty-four Wistar rats with 30% total body surface area (TBSA) full-thickness burn were randomly divided into enteral nutrition (EN, n=32) group and enteral immuno-nutrition (EIN, n=32) group. Another 8 Wistar rats were used as normal control group. The concentration of secretory immunoglobulin A (sIgA) in intestinal mucosa, the blood levels of diamine oxidase (DAO) activity and tumor necrosis factor-alpha (TNF-alpha) were determined, and the expression of TNF-alpha mRNA in intestinal mucosa was determined by reverse transcription polymerase chain reaction (PT-PCR) in control group and on 1st, 4th, 7th, and 10th day postburn in EN and EIN group.
Compared with that of before injury, the blood levels of DAO activity, TNF-alpha content, the concentration of sIgA and the expression of TNF-alpha mRNA in intestinal mucosa were significantly increased in both EN and EIN groups (P<0.05 or P<0.01). However, the blood levels DAO activity, TNF-alpha content, the concentration of sIgA on 4th, 7th and 10th postinjury days and the expression of TNF-alpha mRNA in intestinal mucosa on 1st, 4th, 7th and 10th days postinjury in EIN group were significantly lower than those in EN group (P<0.05 or P<0.01).
EIN can ameliorate the damage of the intestinal mucosa in burned rats compared with EN group. This might be related to an increase in the sIgA level and reduction of the expression of TNF-alpha mRNA in intestinal mucosa.
探讨肠内免疫营养对严重烧伤后肠黏膜损伤的保护作用。
将64只烧伤总面积达30%体表面积（TBSA）的Wistar大鼠随机分为肠内营养（EN，n = 32）组和肠内免疫营养（EIN，n = 32）组。另取8只Wistar大鼠作为正常对照组。测定对照组以及EN组和EIN组烧伤后第1、4、7和10天肠黏膜中分泌型免疫球蛋白A（sIgA）的浓度、血液中二胺氧化酶（DAO）活性及肿瘤坏死因子-α（TNF-α）水平，并采用逆转录聚合酶链反应（PT-PCR）检测肠黏膜中TNF-α mRNA的表达。
与伤前相比，EN组和EIN组血液中DAO活性、TNF-α含量、肠黏膜中sIgA浓度及TNF-α mRNA表达均显著升高（P<0.05或P<0.01）。然而，EIN组伤后第4、7和10天血液中DAO活性、TNF-α含量、sIgA浓度以及伤后第1、4、7和10天肠黏膜中TNF-α mRNA表达均显著低于EN组（P<0.05或P<0.01）。
与EN组相比，EIN可减轻烧伤大鼠肠黏膜的损伤。这可能与肠黏膜中sIgA水平升高及TNF-α mRNA表达降低有关。
