Toyota Central Research and Development Labs., Inc., 41-1 Yokomichi, Nagakute, Aichi 480-1192, Japan.
J Biosci Bioeng. 2010 Sep;110(3):359-62. doi: 10.1016/j.jbiosc.2010.02.012. Epub 2010 Mar 16.
We have studied the effect of enhanced oxygen delivery by perfluorocarbons on the differentiation of C2C12 cells. The extent of differentiation was assessed by means of phase contrast/fluorescence microscopy, active tension measurement and the glucose consumption/lactate production rates. We found that enhanced oxygen delivery is suitable for full differentiation of C2C12 cells.
我们研究了通过全氟化碳增强氧气输送对 C2C12 细胞分化的影响。通过相差/荧光显微镜、主动张力测量和葡萄糖消耗/乳酸产生速率来评估分化程度。我们发现,增强氧气输送适合 C2C12 细胞的完全分化。