Brelińska R, Seidel H J, Kreja L
Department of Histology and Embryology, Medical School, Poznań, Poland.
Cell Tissue Res. 1991 Apr;264(1):175-83. doi: 10.1007/BF00305736.
Thymic nurse cell complexes (TNC-c) were isolated from thymuses of BDF1 mice at pre-determined intervals during the 12-week latency period that precedes the development of leukemias. T-cell leukemias were induced by a single i.v. injection of 50 mg/kg of methylnitrosourea (MNU). In order to clarify processes taking place in TNC-c, the complexes of mice after MNU injection were compared with TNC-c of age-matched control mice, with respect to their number per thymus, the distribution of TNC-c according to their size (the number of intra-TNC thymocytes reflects the type of TNC-c), the number of intra-TNC thymocytes that undergo DNA synthesis, and the phenotype of thymocytes inside TNC-c. During the latency period of leukemogenesis, the effects of MNU were shown to involve, in addition to changes in number of TNC-c, a decrease in the number of thymocytes incorporating labeled thymidine, viz., the number of dividing cells, thus affecting the size distribution of TNC-c types. Intra-TNC thymocytes of control mice were heterogeneous in their phenotype and represented cells at varying stages of their maturation cycle. MNU administration was followed by selective differentiation of thymocytes within TNC-c to Lyt 1-thymocytes in some and to Lyt 2-thymocytes in others. Lyt 1 and Lyt 2 being specific antigens expressed by thymocytes.
在白血病发生前12周的潜伏期内，于预定时间点从BDF1小鼠的胸腺中分离出胸腺哺育细胞复合体（TNC-c）。通过静脉内单次注射50 mg/kg的甲基亚硝基脲（MNU）诱导T细胞白血病。为了阐明TNC-c中发生的过程，将注射MNU后的小鼠的复合体与年龄匹配的对照小鼠的TNC-c在每个胸腺中的数量、TNC-c按大小的分布（TNC内胸腺细胞的数量反映TNC-c的类型）、进行DNA合成的TNC内胸腺细胞的数量以及TNC-c内胸腺细胞的表型方面进行了比较。在白血病发生的潜伏期内，已表明MNU的作用除了涉及TNC-c数量的变化外，还包括掺入标记胸腺嘧啶核苷的胸腺细胞数量减少，即分裂细胞的数量减少，从而影响TNC-c类型的大小分布。对照小鼠的TNC内胸腺细胞在表型上是异质的，代表处于其成熟周期不同阶段的细胞。给予MNU后，TNC-c内的胸腺细胞在一些情况下选择性分化为Lyt 1胸腺细胞，在另一些情况下分化为Lyt 2胸腺细胞。Lyt 1和Lyt 2是胸腺细胞表达的特异性抗原。
