Milić Mirta, Rozgaj Ružica, Kašuba Vilena, Oreščanin Višnja, Balija Melita, Jukić Irena
Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Acta Biochim Pol. 2010;57(3):339-45. Epub 2010 Jun 11.
The aim of this study was to find correlations between folate and vitamin B₁₂ on baseline damage in white blood cells and their association with smoking, alcohol consumption and ageing. Thirty-six healthy vitamin non-deficient male subjects were selected in a randomized study. Comet assay (SCGE) and micronucleus (MN) assay were used as biomarkers of DNA damage. The amount of DNA damage was correlated with vitamin B₁₂ and folic acid concentration. Positive, but non-significant correlation (canonical R = 0.61; χ²=28.97; P=0.253) was found between micronucleus (MN) frequency or comet assay parameters (SCGE) and five covariates (age, smoking, alcohol consumption, vitamin B₁₂ and folate blood serum concentration). The highest MN frequency was observed in the group with the lowest vitamin B₁₂ concentration (F=3.59; P=0.024). The SCGE assay failed to show significant correlation with vitamin B₁₂ or folic acid concentration. Concentration of vitamin B₁₂ was significantly correlated with incidence of micronuclei. Our results present background data that could be valuable for future genotoxicological monitoring.
本研究的目的是找出叶酸和维生素B₁₂与白细胞基线损伤之间的相关性，以及它们与吸烟、饮酒和衰老的关联。在一项随机研究中选取了36名健康且维生素不缺乏的男性受试者。彗星试验（单细胞凝胶电泳，SCGE）和微核试验（MN）被用作DNA损伤的生物标志物。DNA损伤量与维生素B₁₂和叶酸浓度相关。在微核（MN）频率或彗星试验参数（SCGE）与五个协变量（年龄、吸烟、饮酒、维生素B₁₂和叶酸血清浓度）之间发现了正相关，但无显著相关性（典型相关系数R = 0.61；χ²=28.97；P=0.253）。在维生素B₁₂浓度最低的组中观察到最高的微核频率（F=3.59；P=0.024）。单细胞凝胶电泳试验未能显示出与维生素B₁₂或叶酸浓度有显著相关性。维生素B₁₂浓度与微核发生率显著相关。我们的结果提供了背景数据，可能对未来的遗传毒理学监测有价值。
