Mantsch H H, McElhaney R N
Institute of Molecular Science, National Research Council of Canada, Ottawa, Ontario.
Chem Phys Lipids. 1991 Mar;57(2-3):213-26. doi: 10.1016/0009-3084(91)90077-o.
Fourier transform infrared (FT-IR) spectroscopy is an extremely powerful yet non-perturbing physical technique for monitoring the conformation and dynamics of all portions of the phospholipid molecule. In this brief review we summarize some recent FT-IR spectroscopic studies of phospholipid phase transitions in model lipid bilayer and in biological membranes which illustrate the great utility of this technique. We show that FT-IR spectroscopy can accurately monitor the gel to liquid-crystalline phase transition and can provide a large amount of detailed information about phospholipid structure and organization in both the gel and liquid-crystalline states of lipid bilayers.
傅里叶变换红外（FT-IR）光谱法是一种极其强大且无干扰的物理技术，可用于监测磷脂分子各部分的构象和动力学。在这篇简短的综述中，我们总结了一些最近利用FT-IR光谱法对模型脂质双层和生物膜中磷脂相变进行的研究，这些研究说明了该技术的巨大实用性。我们表明，FT-IR光谱法可以准确监测凝胶态到液晶态的相变，并能提供关于脂质双层在凝胶态和液晶态下磷脂结构和组织的大量详细信息。
