Department of Gastroenterology, Pomeranian Medical University, Szczecin, Poland.
Int J Colorectal Dis. 2010 Sep;25(9):1079-85. doi: 10.1007/s00384-010-0980-z. Epub 2010 Jun 12.
We aimed to study the intracellular expression of 5-lipoxygenase (5-LOX), the primary competitor with cyclooxygenase-2 in arachidonic acid metabolism, as inflammatory enzymes may be involved in blocking apoptosis and promoting cancer growth by changing arachidonic acid metabolism within cells. Our purpose was to investigate the possible connection between 5-LOX expression and colon carcinogenesis by characterizing 5-LOX expression in histologically different colonic adenomas, determining the relationship between high expression of 5-LOX and various conventional clinicopathological features of adenomas, and finally characterizing the histological localization of cells with 5-LOX overexpression.
A total of 111 patients were examined and 120 histologically different colonic adenomas analyzed (including four cases of intramucosal adenocarcinoma in a polyp). Immunohistochemical staining with polyclonal anti-5-LOX antibodies was performed.
There was a significant correlation between high 5-LOX expression and patient age, increased polyp size, high grade of intraepithelial neoplasia, villous and tubulovillous adenoma, and histological epithelial localization.
We observed a strong positive correlation between 5-LOX overexpression and the appearance of typical high-risk factors for malignant transformation in adenomatous polyps. The results support the role of 5-LOX in early stages of colon carcinogenesis.
我们旨在研究 5-脂氧合酶（5-LOX）的细胞内表达，5-LOX 是花生四烯酸代谢中与环氧化酶-2 的主要竞争酶，因为炎症酶可能通过改变细胞内的花生四烯酸代谢而参与阻止细胞凋亡和促进癌症生长。我们的目的是通过研究组织学不同的结肠腺瘤中 5-LOX 的表达，确定高表达 5-LOX 与腺瘤的各种常规临床病理特征之间的关系，最后描述具有 5-LOX 过表达的细胞的组织学定位，来研究 5-LOX 表达与结肠癌变之间的可能联系。
共检查了 111 例患者，分析了 120 个组织学不同的结肠腺瘤（包括 4 例息肉内黏膜内腺癌）。用多克隆抗 5-LOX 抗体进行免疫组织化学染色。
高 5-LOX 表达与患者年龄、息肉增大、上皮内瘤变程度增加、绒毛状和管状绒毛状腺瘤以及组织学上皮定位之间存在显著相关性。
我们观察到 5-LOX 过表达与腺瘤中典型的恶变高危因素之间存在强烈的正相关。这些结果支持 5-LOX 在结直肠癌发生的早期阶段的作用。
