Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
Planta. 2010 Aug;232(3):677-89. doi: 10.1007/s00425-010-1199-5. Epub 2010 Jun 13.
A combination of enzyme mapping, FT-IR microscopy and NMR spectroscopy was used to study temporal and spatial aspects of endosperm cell wall synthesis and deposition in developing grain of bread wheat cv. Hereward. This confirmed previous reports that changes in the proportions of the two major groups of cell wall polysaccharides occur, with beta-glucan accumulating earlier in development than arabinoxylan. Changes in the structure of the arabinoxylan occurred, with decreased proportions of disubstituted xylose residues and increased proportions of monosubstituted xylose residues. These are likely to result, at least in part, from arabinoxylan restructuring catalysed by enzymes such as arabinoxylan arabinofurano hydrolase and lead to changes in cell wall mechanical properties which may be required to withstand stresses during grain maturation and desiccation.
采用酶谱分析、傅里叶变换红外显微镜和核磁共振波谱技术，研究了面包小麦品种 Hereward 发育籽粒中胚乳细胞壁合成和沉积的时空特征。这证实了先前的报告，即细胞壁多糖的两个主要群的比例发生变化，β-葡聚糖的积累比阿拉伯木聚糖更早。阿拉伯木聚糖的结构发生变化，二取代木糖残基的比例降低，单取代木糖残基的比例增加。这些变化可能至少部分是由阿拉伯木聚糖阿拉伯呋喃糖苷水解酶等酶催化的阿拉伯木聚糖重构引起的，导致细胞壁机械性能的变化，这可能是籽粒成熟和干燥过程中抵抗应力所必需的。
