Jamme F, Robert P, Bouchet B, Saulnier L, Dumas P, Guillon F
Synchrotron SOLEIL, L'Orme des Merisiers, BP 48, Saint Aubin, F-91192, Gif-sur-yvette, France.
Appl Spectrosc. 2008 Aug;62(8):895-900. doi: 10.1366/000370208785284448.
Infrared microspectroscopy and immunolabeling techniques were employed in order to obtain deeper insight into the biochemical nature of aleurone cell walls of wheat grain. The use of a synchrotron source, thanks to its intrinsic brightness, has provided unprecedented information at the level of a few micrometers and has allowed the discrimination of various polysaccharides in cell walls. The high spectral quality obtained in the small analyzed domain has been beneficial in estimating the relative proportions of beta-glucan and arabinoxylan, through the use of principal component analysis (PCA). The highest amount of beta-glucan is found in periclinal cell walls close to the starchy endosperm. The junction regions between aleurone cells are enriched in arabinoxylan. At the early stage of wheat grain development (271 degrees D), the chemical composition along the cell walls is more heterogeneous than at the mature stage. Both synchrotron infrared microspectroscopy and immunolabeling experiments made it possible to reveal the spatial heterogeneity of the various chemical compositions of aleurone cell walls.
为了更深入地了解小麦籽粒糊粉层细胞壁的生化性质，采用了红外显微光谱和免疫标记技术。由于同步辐射源本身的亮度，其在几微米的尺度上提供了前所未有的信息，并能够区分细胞壁中的各种多糖。通过主成分分析（PCA），在小分析区域获得的高光谱质量有助于估计β-葡聚糖和阿拉伯木聚糖的相对比例。在靠近淀粉胚乳的周缘细胞壁中发现了最高含量的β-葡聚糖。糊粉层细胞之间的连接区域富含阿拉伯木聚糖。在小麦籽粒发育的早期阶段（271度日），细胞壁的化学成分比成熟阶段更加不均匀。同步辐射红外显微光谱和免疫标记实验都揭示了糊粉层细胞壁各种化学成分的空间异质性。
