Instituto Dermatológico de Jalisco "Dr. José Barba Rubio"Secretaría de Salud Jalisco, Guadalajara, Jalisco, Mexico.
Clin Rheumatol. 2010 Sep;29(9):1055-9. doi: 10.1007/s10067-010-1515-2. Epub 2010 Jun 14.
Morphea is a disease that affects connective tissue and microvessels. Its pathogenesis is unknown, but several autoimmune factors participate. Our objective was to determine the frequency of antinuclear antibodies (ANAs) in pediatric patients with morphea and to establish their relation with the clinical variants and disease activity. A cross-sectional study was carried out from January 1999 to January 2008 in patients with morphea seen at the Instituto Dermatologico de Jalisco. ANAs were determined through an indirect immunoflourescent method, and the immunospecificity was done with a double immunodiffusion technique in agarose gel. A total of 34 children were included in the study, 74% of the female gender. Plaque morphea was the most common variant, present in 44% of the cases, followed by linear morphea in 38%, and generalized morphea in 18%. ANAs were positive in 29%, with homogenous immunoflourescense as the most frequent pattern (70%). Of the ANA-positive patients, 83% had generalized morphea, and in 70% of the cases the disease were considered as active. The frequency of ANA-positive children with morphea was 29%, and seems to be related to more extensive disease. No previous studies exist on this topic in the mestizo Mexican population.
硬斑病是一种影响结缔组织和微血管的疾病。其发病机制尚不清楚，但有几个自身免疫因素参与。我们的目的是确定硬斑病患儿抗核抗体（ANA）的频率，并确定其与临床变异型和疾病活动度的关系。这是一项从 1999 年 1 月至 2008 年 1 月在哈利斯科州皮肤病学研究所就诊的硬斑病患者中进行的横断面研究。通过间接免疫荧光法测定 ANA，并用琼脂糖凝胶中的双免疫扩散技术测定免疫特异性。共有 34 名儿童纳入研究，其中 74%为女性。斑块硬斑病是最常见的变异型，占 44%，其次是线状硬斑病占 38%，全身性硬斑病占 18%。ANA 阳性率为 29%，以均质免疫荧光为最常见的模式（70%）。ANA 阳性的患者中，83%为全身性硬斑病，70%的病例被认为是活动期。硬斑病患儿 ANA 阳性率为 29%，似乎与更广泛的疾病有关。在墨西哥梅斯蒂索人群中，以前没有关于这个主题的研究。
