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利用同工酶标记评估湄公河野鲮(Labeo calbasu)地理种群的遗传分化。

Assessing genetic differentiation in geographic populations of Labeo calbasu using allozyme markers.

机构信息

National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, UP, India.

出版信息

Biochem Genet. 2010 Oct;48(9-10):760-78. doi: 10.1007/s10528-010-9358-8. Epub 2010 Jun 13.

DOI:10.1007/s10528-010-9358-8
PMID:20549331
Abstract

The population structure of Labeo calbasu from 11 rivers belonging to the Indus, Ganges, Bhima, Mahanadi, and Godavari basins was investigated using allozyme marker systems. Seven out of 20 allozyme loci (35%) were polymorphic (P < 0.99). Both probability and score tests indicated significant deviation of genotype proportions from Hardy-Weinberg expectations at two loci, XDH* (Mahanadi, Bhima, and Godavari) and G6PDH* (Mahanadi). A pairwise genetic homogeneity test and F (ST) values indicated a low-to-moderate level (0.0515) of genetic structuring in the wild population of L. calbasu. AMOVA analysis also indicated moderate differentiation among the samples from different river basins. Analysis for genetic bottleneck was performed under the infinite allele model. The study revealed nine genetic stocks of L. calbasu from the natural population across Indian rivers. Evidence of genetic bottlenecks in some rivers was also revealed.

摘要

采用同工酶标记系统研究了来自印度河、恒河、比马河、马哈纳迪河和戈达瓦里河流域的 11 条河流的拟鲤种群结构。在 20 个同工酶基因座中,有 7 个(35%)表现出多态性(P<0.99)。概率和得分检验均表明,在两个基因座(Mahanadi、Bhima 和 Godavari 的 XDH和 Mahanadi 的 G6PDH)的基因型比例明显偏离哈迪-温伯格期望。成对遗传同质性检验和 F(ST)值表明,在拟鲤野生种群中存在低到中等水平(0.0515)的遗传结构。AMOVA 分析还表明,来自不同河流流域的样本存在中度分化。在无限等位基因模型下进行遗传瓶颈分析。研究揭示了来自印度河流域自然种群的 9 个拟鲤遗传种群。一些河流中也存在遗传瓶颈的证据。

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