野生印度野鲮（Hamilton，1822）线粒体ATPase 6/8基因的特征分析及自然遗传多样性图谱绘制。
Characterization of mitochondrial ATPase 6/8 genes in wild Labeo calbasu (Hamilton, 1822) and mapping of natural genetic diversity.
作者信息
Singh Rajeev K, Lal Kuldeep K, Mohindra Vindhya, Sah Rama S, Kumar Rajesh, Jena J K
机构信息
a Fish Conservation Division, NBFGR , Lucknow , UP , India.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3078-81. doi: 10.3109/19401736.2014.1003917. Epub 2015 Jan 29.
We characterized mitochondrial ATP synthase (ATPase) 6 and 8 genes in Labeo calbasu (Hamilton, 1822) and determined genetic variation in wild populations across the natural distribution in Indian rivers. A total of 206 individuals were sampled from 11 riverine sites belonging to distinct geographical locations covering five major river basins. Sequencing of 842 base pairs of ATPase 6/8 revealed 21 haplotypes with haplotype diversity ranging from 0.1250 (River Satluj) to 0.8846 (River Bhagirathi). Analysis of molecular variance (AMOVA) of mitochondrial DNA (mtDNA) data revealed significant genetic differentiation among sites (FST = 0.192, p < 0.0001) which was indicative of moderate level of genetic structuring in the wild L. calbasu populations. The patterns of genetic divergence and haplotype network of mtDNA revealed distinct clades present in Indian rivers. The analysis of data demonstrated the potential of ATPase 6/8 genes in determining the genetic diversity and indicated considerable sub-structuring in wild calbasu populations present in different rivers.
我们对印度野鲮（Labeo calbasu，汉密尔顿，1822年）的线粒体ATP合酶（ATPase）6和8基因进行了特征分析，并确定了印度河流自然分布范围内野生种群的遗传变异。总共从属于不同地理位置、覆盖五个主要流域的11个河流站点采集了206个个体样本。对ATPase 6/8的842个碱基对进行测序，发现了21种单倍型，单倍型多样性范围从0.1250（萨特卢杰河）到0.8846（巴吉拉蒂河）。线粒体DNA（mtDNA）数据的分子方差分析（AMOVA）显示，各站点之间存在显著的遗传分化（FST = 0.192，p < 0.0001），这表明野生印度野鲮种群存在中等程度的遗传结构。mtDNA的遗传分化模式和单倍型网络显示，印度河流中存在不同的进化枝。数据分析表明，ATPase 6/8基因在确定遗传多样性方面具有潜力，并表明不同河流中的野生印度野鲮种群存在相当程度的亚结构。
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