Department of Pharmacology, University of Pennsylvania Medical School-Pharmacology, 36th and Hamilton Walk, Philadelphia, PA 19104, USA.
Anal Bioanal Chem. 2010 Aug;397(8):3173-8. doi: 10.1007/s00216-010-3821-6. Epub 2010 Jun 13.
Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microscope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.
转染是一种强大的分析工具，可用于研究细胞中基因和基因产物的功能。转染方法广泛分为生物、化学和物理三类。这些方法已经得到了改进，现在可以使用精确控制的激光显微镜系统将核酸递送到细胞的特定亚细胞区域。定点转染和 mRNA 转染的结合是研究基因和基因产物功能的新方法。然而，每种方法都有其优点和缺点，因此最佳方法取决于实验设计和目标。
