Institute of Food Technology and Bioprocess Engineering, Technical University Dresden, 01062 Dresden, Germany.
Bioprocess Biosyst Eng. 2011 Jan;34(1):21-31. doi: 10.1007/s00449-010-0443-5. Epub 2010 Jun 15.
The dimorphic yeasts Candida boidinii and Yarrowia lipolytica were applied as model organisms to study mycelial growth. A mathematical model of hybrid cellular automaton type was developed to analyze the impact of different biological assumptions on the predicted development of filamentous yeast colonies. The one-dimensional model described discrete cells and continuous distribution of nutrients. The simulation algorithm accounted for proliferation of cells, diffusion of nutrient, as well as biomass decay and recycling inside the mycelium. Simulations reproduced the spatio-temporal development of C. boidinii colonies when a diffusion-limited growth algorithm based on the growth of pseudohyphal cells was applied. Development of Y. lipolytica colonies could only be reproduced when proliferation was restricted to the colony boundary, and cell decay and biomass recycling were incorporated into the model. The results suggested that cytoplasm, which served as the secondary nutrient resource, had to be translocated inside the hyphal network.
双相酵母 Candida boidinii 和 Yarrowia lipolytica 被用作模式生物来研究菌丝体生长。开发了一种混合细胞自动机类型的数学模型，以分析不同生物学假设对预测丝状酵母菌落发育的影响。一维模型描述了离散的细胞和连续的营养物质分布。模拟算法考虑了细胞的增殖、营养物质的扩散，以及菌丝体内的生物量衰减和再循环。当应用基于假菌丝细胞生长的扩散限制生长算法时，模拟再现了 C. boidinii 菌落的时空发展。只有当增殖仅限于菌落边界，并将细胞衰减和生物量再循环纳入模型时，才能再现 Y. lipolytica 菌落的发展。结果表明，细胞质作为二次营养资源，必须在菌丝网络内部转运。
