Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing, Jiangsu, China.
Environ Toxicol. 2012 Jan;27(1):50-7. doi: 10.1002/tox.20612. Epub 2010 May 26.
Organophosphates and carbamates are widely used pesticides and play an important role in global agriculture. The misuse of these compounds has caused environmental problems and has had a negative impact on wildlife. In this study, the in vivo effects of commercial chlorpyrifos and isoprocarb on acetylcholinesterase (AChE) and glutathione S-transferase (GST) activities in goldfish (Carassius auratus) were investigated. Muscle and brain AChE activity was significantly inhibited by chlorpyrifos and isoprocarb (alone and in combination) after 2, 5, 10, and 15 days of exposure, and obvious concentration-response and time-response relationships were obtained. Gill GST activity was significantly inhibited by chlorpyrifos and isoprocarb (single compounds and in combination), however, concentration dependence and time dependence were not apparent. The joint effect of chlorpyrifos/isoprocarb was additive with regard to AChE activity inhibition and was antagonistic with regard to GST activity inhibition.
有机磷和氨基甲酸酯类农药被广泛应用，在全球农业中发挥着重要作用。这些化合物的滥用造成了环境问题，并对野生动物产生了负面影响。本研究旨在探讨商业用毒死蜱和异丙威对金鱼（Carassius auratus）乙酰胆碱酯酶（AChE）和谷胱甘肽 S-转移酶（GST）活性的体内影响。结果表明，在暴露 2、5、10 和 15 天后，肌肉和脑 AChE 活性被毒死蜱和异丙威（单独和联合）显著抑制，且呈现明显的浓度-反应和时间-反应关系。鳃 GST 活性也被毒死蜱和异丙威（单一化合物和混合物）显著抑制，但没有明显的浓度依赖性和时间依赖性。在 AChE 活性抑制方面，毒死蜱/异丙威的联合作用为相加作用，而在 GST 活性抑制方面为拮抗作用。
