Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environmental Science and Engineering, Hohai University, Nanjing 210098, People's Republic of China.
Environ Toxicol Pharmacol. 2009 Nov;28(3):414-9. doi: 10.1016/j.etap.2009.07.005. Epub 2009 Jul 14.
In the present study, the in vivo effects of commercial propoxur alone and in combination with isoprocarb and chlorpyrifos on acetylcholinesterase and glutathione S-transferases activities in goldfish (Carassius auratus) were investigated. Brain and muscle AChE activity was significantly inhibited (88% and 85% at most, respectively) by propoxur after 5 d of exposure at concentrations between 141 and 2263μg/L, bell-shaped concentration-response curves were obtained. In most cases, time dependence was apparent. Under insecticide mixture exposure (binary mixture propoxur/isoprocarb and ternary mixture propoxur/isoprocarb/chlorpyrifos), the inhibition of AChE activity was significant in all cases and linear concentration-response relationships were observed. Propoxur (alone and in combination) significantly inhibited gill GST activity, and both mixtures also induced significant inhibition of liver GST activity. However, concentration and time dependence were not apparent. AChE is likely to be more informative than GST and seems to be a good biomarker to diagnose exposure to anticholinesterase pesticides in wild populations of this species.
在本研究中，研究了单独使用和与异丙威和毒死蜱联合使用的商业丙硫磷对金鱼（Carassius auratus）乙酰胆碱酯酶和谷胱甘肽 S-转移酶活性的体内影响。暴露于 141 至 2263μg/L 浓度的丙硫磷 5 天后，脑和肌肉 AChE 活性显著抑制（分别最多 88%和 85%），得到钟形浓度-反应曲线。在大多数情况下，时间依赖性明显。在杀虫剂混合物暴露（丙硫磷/异丙威二元混合物和丙硫磷/异丙威/毒死蜱三元混合物）下，所有情况下 AChE 活性的抑制均很明显，并且观察到线性浓度-反应关系。丙硫磷（单独和组合）显著抑制了鳃 GST 活性，两种混合物也诱导了肝 GST 活性的显著抑制。然而，浓度和时间依赖性不明显。AChE 可能比 GST 更具信息量，并且似乎是诊断该物种野生种群暴露于抗胆碱酯酶农药的良好生物标志物。
