Sublethal effects of pesticide mixtures on selected biomarkers of Carassius auratus.

In the present study, the in vivo effects of commercial propoxur alone and in combination with isoprocarb and chlorpyrifos on acetylcholinesterase and glutathione S-transferases activities in goldfish (Carassius auratus) were investigated. Brain and muscle AChE activity was significantly inhibited (88% and 85% at most, respectively) by propoxur after 5 d of exposure at concentrations between 141 and 2263μg/L, bell-shaped concentration-response curves were obtained. In most cases, time dependence was apparent. Under insecticide mixture exposure (binary mixture propoxur/isoprocarb and ternary mixture propoxur/isoprocarb/chlorpyrifos), the inhibition of AChE activity was significant in all cases and linear concentration-response relationships were observed. Propoxur (alone and in combination) significantly inhibited gill GST activity, and both mixtures also induced significant inhibition of liver GST activity. However, concentration and time dependence were not apparent. AChE is likely to be more informative than GST and seems to be a good biomarker to diagnose exposure to anticholinesterase pesticides in wild populations of this species.