Abbal M, Tkaczuk J, Praud C, Msayeh F, Ohayon E
Laboratoire Central d'Immunologie, CHU Toulouse Rangueil, France.
Complement Inflamm. 1991;8(2):92-103. doi: 10.1159/000463185.
Using apparatus available in any laboratory we developed a semiautomated kinetic technique for complement activity assay. Hemolysis of sensitized red blood cells is performed in the thermostated microflow cell of a spectrophotometer connected to a computer. The computer controls, displays on the screen, and analyzes all the different phases of the assay. After definition of optimal operating conditions, we compared the results obtained by this technique and by Kabat and Mayer's. On 221 patients' sera the regression coefficient was 0.94. The values for samples deficient in one fraction or after in vitro activation were very similar. The coefficient of variation was close to 1% for within series studies and better than 3% between series. This technique is very easy to perform even in a routine nonspecialized laboratory and up to 30-40 sample/h can be tested.
我们利用任何实验室都具备的仪器开发了一种用于补体活性测定的半自动动力学技术。在连接到计算机的分光光度计的恒温微流池中进行致敏红细胞的溶血反应。计算机控制、在屏幕上显示并分析测定的所有不同阶段。在确定最佳操作条件后，我们比较了该技术与卡巴特和迈耶方法所获得的结果。对221份患者血清进行检测，回归系数为0.94。对于缺乏某一组分的样本或体外激活后的样本，其值非常相似。同组研究的变异系数接近1%，组间变异系数优于3%。即使在常规的非专业实验室中，该技术也非常易于操作，每小时最多可检测30 - 40个样本。
