Medina R F, Rondon S I, Reyna S M, Dickey A M
Texas A&M, Department of Entomology, College Station, TX 77843-2475, USA.
Environ Entomol. 2010 Jun;39(3):1037-42. doi: 10.1603/EN09286.
The potato tuberworm, Phthorimaea operculella Zeller, is one of the most damaging pests of potatoes worldwide. Although tuberworm was recorded in California as early as 1856, it was first reported in damaging numbers in Oregon and Washington in the early 2000s. The objective of this study was to provide baseline information on the population structure of potato tuberworm in the United States. Potato tuberworm adults were collected from potato fields in the major production regions in the United States. Amplified fragment length polymorphisms (AFLP) were used to determine the genetic population structure of potato tuberworm. We used 219 individuals and 335 polymorphic AFLP bands to infer the geographic population structure of potato tuberworm. Posterior probabilities calculated using the model-based clustering method implemented in STRUCTURE, and F(st) values calculated using AFLP-SURV confirmed the existence of two distinct populations in the United States (one mostly associated to the western United States and the other one mostly associated to the eastern United States). This study provides baseline data for the molecular characterization of potato tuberworm populations, which will aid in tracking the origin of future invasions within the United States.
马铃薯块茎蛾（Phthorimaea operculella Zeller）是全球对马铃薯危害最大的害虫之一。尽管早在1856年加利福尼亚就有马铃薯块茎蛾的记录，但21世纪初它才首次在俄勒冈州和华盛顿州造成大量危害的报道。本研究的目的是提供美国马铃薯块茎蛾种群结构的基础信息。从美国主要产区的马铃薯田采集了马铃薯块茎蛾成虫。利用扩增片段长度多态性（AFLP）来确定马铃薯块茎蛾的遗传种群结构。我们使用219个个体和335个多态性AFLP条带推断马铃薯块茎蛾的地理种群结构。使用STRUCTURE中基于模型的聚类方法计算的后验概率以及使用AFLP-SURV计算的F(st)值证实了美国存在两个不同的种群（一个主要与美国西部相关，另一个主要与美国东部相关）。本研究为马铃薯块茎蛾种群的分子特征提供了基础数据，这将有助于追踪美国境内未来入侵的源头。
