利用在体麻醉禽类胚胎进行无运动伪影的活体成像。
Motion-artifact-free in vivo imaging utilizing narcotized avian embryos in ovo.
机构信息
Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), Beutenbergstrasse 11a, 07745 Jena, Germany.
出版信息
Mol Imaging Biol. 2011 Apr;13(2):208-14. doi: 10.1007/s11307-010-0355-4.
PURPOSE
The chick embryo in ovo is a well-accessible and economical in vivo model, but its use in molecular imaging has been limited because of motion artifacts on resulting images. The purpose of this study was to develop a method using narcotics to inhibit motility and to perform motion-artifact-free imaging of living chick embryos in ovo.
PROCEDURES
Chick embryos in ovo were narcotized using three different narcotics: isoflurane, 2,2,2-tribromoethanol, and urethane/α-chloralose. Narcotized embryos were imaged using micro-computed tomography (microCT) at days 10-18 of incubation, and the resulting images were analyzed for reduction of motion artifacts.
RESULTS
All three anesthetics could be used for anesthetizing living chick embryos in ovo thus allowing the acquisition of motion-artifact-free images.
CONCLUSIONS
Our experiments revealed that isoflurane is the best-suited narcotic for single and repeated applications to image living chick embryos in ovo.
目的
鸡胚在卵内是一种易于接近且经济的体内模型，但由于图像上存在运动伪影，其在分子成像中的应用受到限制。本研究的目的是开发一种使用麻醉剂抑制运动的方法，并对在卵内的活体鸡胚进行无运动伪影的成像。
方法
使用三种不同的麻醉剂（异氟烷、2,2,2-三溴乙醇和氨基甲酸乙酯/α-氯醛）对在卵内的鸡胚进行麻醉。对孵化第 10-18 天的麻醉胚胎进行微计算机断层扫描（microCT）成像，并对所得图像进行运动伪影减少的分析。
结果
三种麻醉剂均可用于麻醉在卵内的活体鸡胚，从而可以获得无运动伪影的图像。
结论
我们的实验表明，异氟烷最适合单次和重复应用于在卵内成像的活体鸡胚。
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