视网膜色素上皮细胞双视黄醛脂褐质发色团A2E的实验研究方法
Experimental approaches to the study of A2E, a bisretinoid lipofuscin chromophore of retinal pigment epithelium.
作者信息
Sparrow Janet R, Kim So Ra, Wu Yalin
机构信息
Departments of Ophthalmology and Pathology and Cell Biology, Columbia University, New York, NY 10032, USA.
出版信息
Methods Mol Biol. 2010;652:315-27. doi: 10.1007/978-1-60327-325-1_18.
Abstract
Bisretinoid lipofuscin compounds that accumulate in retinal pigment epithelial (RPE) cells are implicated in the pathogenesis of some forms of macular degeneration. In the development of approaches to the amelioration of retinal disorders characterized by enhanced RPE lipofuscin formation, attention is being given to therapies that reduce the production of these damaging pigments. An understanding of the biosynthetic pathways by which these molecules form is essential to the development of these therapies. Thus methods for studying the biosynthesis of these compounds are presented. A tissue culture model is also described whereby a human RPE cell line that is otherwise devoid of bisretinoid lipofuscin compounds is employed and synthesized A2E is delivered to the cells. This approach allows for a population of RPE cells that have accumulated the lipofuscin fluorophore A2E in addition to A2E-free cells.
摘要
在视网膜色素上皮（RPE）细胞中积累的双视黄醛脂褐素化合物与某些形式的黄斑变性的发病机制有关。在开发改善以RPE脂褐素形成增强为特征的视网膜疾病的方法时，人们将注意力集中在减少这些有害色素产生的疗法上。了解这些分子形成的生物合成途径对于开发这些疗法至关重要。因此，本文介绍了研究这些化合物生物合成的方法。还描述了一种组织培养模型，即使用原本不含双视黄醛脂褐素化合物的人RPE细胞系，并将合成的A2E递送至细胞。这种方法可以产生一群除了不含A2E的细胞外还积累了脂褐素荧光团A2E的RPE细胞。
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本文引用的文献
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J Biol Chem. 2009 Jul 24;284(30):20155-66. doi: 10.1074/jbc.M109.021345. Epub 2009 May 28.
2
The age lipid A2E and mitochondrial dysfunction synergistically impair phagocytosis by retinal pigment epithelial cells.衰老脂质A2E与线粒体功能障碍协同损害视网膜色素上皮细胞的吞噬作用。
J Biol Chem. 2008 Sep 5;283(36):24770-80. doi: 10.1074/jbc.M800706200. Epub 2008 Jul 10.
3
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Exp Eye Res. 2008 Jun;86(6):975-82. doi: 10.1016/j.exer.2008.03.016. Epub 2008 Mar 28.
4
Retinal pigment epithelium lipofuscin proteomics.视网膜色素上皮脂褐素蛋白质组学
Mol Cell Proteomics. 2008 Jul;7(7):1397-405. doi: 10.1074/mcp.M700525-MCP200. Epub 2008 Apr 24.
5
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Adv Exp Med Biol. 2008;613:393-8. doi: 10.1007/978-0-387-74904-4_46.
6
The all-trans-retinal dimer series of lipofuscin pigments in retinal pigment epithelial cells in a recessive Stargardt disease model.隐性Stargardt病模型中视网膜色素上皮细胞脂褐素色素的全反式视黄醛二聚体系列
Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19273-8. doi: 10.1073/pnas.0708714104. Epub 2007 Nov 28.
7
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J Biol Chem. 2005 Dec 2;280(48):39732-9. doi: 10.1074/jbc.M504933200. Epub 2005 Sep 26.
9
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10
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