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蓝光自发荧光眼底成像用于监测皇家外科学院大鼠的视网膜变性

Blue Autofluorescence Fundus Imaging for Monitoring Retinal Degeneration in Royal College of Surgeons Rats.

作者信息

Bubis Ettel, Sher Ifat, Skaat Alon, Sharvit-Ginon Inbal, Szalapak Alicja M, Moroz Iris, Kalter-Leibovici Ofra, Rotenstreich Ygal

机构信息

Goldschleger Eye Institute, Sheba Medical Center, Tel-Hashomer, Israel.

Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.

出版信息

Transl Vis Sci Technol. 2019 Feb 28;8(1):26. doi: 10.1167/tvst.8.1.26. eCollection 2019 Jan.

DOI:10.1167/tvst.8.1.26
PMID:30834174
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6396687/
Abstract

PURPOSE

Development of a method for noninvasive longitudinal follow-up of retinal degeneration in the whole retina for Royal College of Surgeons (RCS) rats, a commonly used model of retinitis pigmentosa associated with mutations in the MER-proto-oncogene tyrosine kinase () gene.

METHODS

Pigmented RCS rats at postnatal (p) days p28 to p84 were subjected to a biweekly spectral-domain optical coherence tomography (SD-OCT), blue laser fundus autofluorescence (BL-FAF) imaging, and multicolor fundus imaging. Wild-type (WT; Long Evans) rats were tested as control.

RESULTS

Hyperautofluorescence developed throughout the fundus at p42, concomitant with a significant increase in SD-OCT thickness and reflectivity of the debris zone (DZ) layer as well as thinning of the photoreceptor outer nuclear layer (ONL). From p56 to p84, discrete hypofluorescent lesions surrounded by hyperfluorescent flecks were demonstrated around the optic disc that gradually spread throughout the retina. The hypofluorescent lesions were associated with loss of ONL and gradual thinning of the DZ layer. No hypofluorescent BL-FAF lesions were observed in WT rats.

CONCLUSIONS

This study suggests that BL-FAF imaging may present a new method for noninvasive longitudinal follow-up of retinal degeneration in nearly the whole retina in RCS rats.

TRANSLATIONAL RELEVANCE

A clinical test was developed that may be implemented in translational studies in the RCS rat model of -associated retinitis pigmentosa.

摘要

目的

开发一种用于对皇家外科学院（RCS）大鼠整个视网膜的视网膜变性进行无创纵向随访的方法，RCS大鼠是一种常用的与MER原癌基因酪氨酸激酶（）基因突变相关的色素性视网膜炎模型。

方法

对出生后（p）28至84天的色素性RCS大鼠每两周进行一次光谱域光学相干断层扫描（SD-OCT）、蓝色激光眼底自发荧光（BL-FAF）成像和多色眼底成像。以野生型（WT；长Evans）大鼠作为对照进行检测。

结果

在p42时整个眼底出现高自发荧光，同时SD-OCT显示碎片带（DZ）层厚度和反射率显著增加，以及光感受器外核层（ONL）变薄。从p56到p84，视盘周围出现由高荧光斑点包围的离散低荧光病变，并逐渐扩散至整个视网膜。这些低荧光病变与ONL的丧失和DZ层的逐渐变薄有关。在WT大鼠中未观察到低荧光的BL-FAF病变。

结论

本研究表明，BL-FAF成像可能为RCS大鼠几乎整个视网膜的视网膜变性无创纵向随访提供一种新方法。

转化相关性

开发了一种临床试验，可在与相关色素性视网膜炎的RCS大鼠模型的转化研究中实施。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/30fac905e24f/i2164-2591-8-1-26-f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/a5f7bec1d048/i2164-2591-8-1-26-f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/c1c0135937e5/i2164-2591-8-1-26-f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/9807c57aa407/i2164-2591-8-1-26-f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/a720b7b66687/i2164-2591-8-1-26-f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/fb58d9b49697/i2164-2591-8-1-26-f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/30fac905e24f/i2164-2591-8-1-26-f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/a5f7bec1d048/i2164-2591-8-1-26-f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/c1c0135937e5/i2164-2591-8-1-26-f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/9807c57aa407/i2164-2591-8-1-26-f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/a720b7b66687/i2164-2591-8-1-26-f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/fb58d9b49697/i2164-2591-8-1-26-f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2ac/6396687/30fac905e24f/i2164-2591-8-1-26-f06.jpg

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