Kanemoto T, Tohya K, Kimura M, Fukuyama A, Kitamura Y
Department of Pathology, Osaka University Medical School, Japan.
Exp Hematol. 1991 May;19(4):288-93.
The effects of culture matrix (agar, collagen, and methylcellulose) on differentiation of mast cells were investigated. Because berberine sulfate-positive colonies in agar were composed of macrophages but not of mast cells, the naphthol AS-D chloroacetate esterase reaction was used to identify mast-cell colonies. When bone marrow cells of WBB6F1 mice were cultured with conditioned media containing interleukin 3 (IL-3), numbers of mast-cell colonies were greater in collagen cultures than in agar and methylcellulose cultures. Electron microscopic examination revealed that mast cells developing in collagen and agar cultures were more mature than those developing in methylcellulose cultures. However, when bone marrow-derived cultured mast cells or purified peritoneal mast cells were plated, the efficiency of colony formation and size of colonies were comparable among agar, collagen, and methylcellulose cultures. Therefore, all three matrices tested had similar effects on the proliferation of mast cells. Collagen appeared to be suitable for differentiation of bone-marrow precursors and their maturation. Agar appeared to be suitable only for maturation.
研究了培养基质（琼脂、胶原蛋白和甲基纤维素）对肥大细胞分化的影响。由于琼脂中硫酸小檗碱阳性集落由巨噬细胞而非肥大细胞组成，因此采用萘酚AS-D氯乙酸酯酶反应来鉴定肥大细胞集落。当用含白细胞介素3（IL-3）的条件培养基培养WBB6F1小鼠的骨髓细胞时，胶原蛋白培养物中肥大细胞集落的数量比琼脂和甲基纤维素培养物中的更多。电子显微镜检查显示，在胶原蛋白和琼脂培养物中发育的肥大细胞比在甲基纤维素培养物中发育的肥大细胞更成熟。然而，当接种骨髓来源的培养肥大细胞或纯化的腹膜肥大细胞时，琼脂、胶原蛋白和甲基纤维素培养物之间的集落形成效率和集落大小相当。因此，所测试的三种基质对肥大细胞增殖具有相似的影响。胶原蛋白似乎适合骨髓前体细胞的分化及其成熟。琼脂似乎仅适合成熟过程。
