Centro Andaluz de Biología del Desarrollo/ CSIC/ Universidad Pablo de Olavide, Carretera de Utrera, Km. 1. 41013 Seville, Spain.
Environ Microbiol. 2010 Jun;12(6):1748-61. doi: 10.1111/j.1462-2920.2010.02254.x.
The CbrAB two-component system has been described in certain species of Pseudomonads as a global regulatory system required for the assimilation of several amino acids (e.g. histidine, proline or arginine) as carbon or carbon and nitrogen sources. In this work, we used global gene expression and phenotypic analyses to characterize the roles of the CbrAB system in Pseudomonas putida. Our results show that CbrB is involved in coordination with the nitrogen control system activator, NtrC, in the uptake and assimilation of several amino acids. In addition, CbrB affects other carbon utilization pathways and a number of apparently unrelated functions, such as chemotaxis, stress tolerance and biofilm development. Based on these new findings, we propose that CbrB is a high-ranked element in the regulatory hierarchy of P. putida that directly or indirectly controls a variety of metabolic and behavioural traits required for adaptation to changing environmental conditions.
CbrAB 双组份系统已在某些假单胞菌物种中被描述为一种全局调控系统，需要该系统来同化几种氨基酸（如组氨酸、脯氨酸或精氨酸）作为碳或碳氮源。在这项工作中，我们使用全局基因表达和表型分析来表征 CbrAB 系统在恶臭假单胞菌中的作用。我们的结果表明，CbrB 参与与氮调控系统激活物 NtrC 的协调，以摄取和同化几种氨基酸。此外，CbrB 还影响其他碳利用途径和许多明显无关的功能，如趋化性、应激耐受和生物膜形成。基于这些新发现，我们提出 CbrB 是恶臭假单胞菌调控层次结构中的一个高级元素，它直接或间接地控制着各种代谢和行为特征，这些特征对于适应不断变化的环境条件是必需的。
