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通过化学水解和工程改造的恶臭假单胞菌实现聚酰胺的升级再造。

Upcycling of polyamides through chemical hydrolysis and engineered Pseudomonas putida.

作者信息

de Witt Jan, Luthe Tom, Wiechert Johanna, Jensen Kenneth, Polen Tino, Wirtz Astrid, Thies Stephan, Frunzke Julia, Wynands Benedikt, Wierckx Nick

机构信息

Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, Jülich, Germany.

Novonesis A/S, Biologiens Vej 2, Kgs, Lyngby, Denmark.

出版信息

Nat Microbiol. 2025 Mar;10(3):667-680. doi: 10.1038/s41564-025-01929-5. Epub 2025 Feb 10.

DOI:10.1038/s41564-025-01929-5
PMID:39929973
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11879879/
Abstract

Aliphatic polyamides, or nylons, are widely used in the textile and automotive industry due to their high durability and tensile strength, but recycling rates are below 5%. Chemical recycling of polyamides is possible but typically yields mixtures of monomers and oligomers which hinders downstream purification. Here, Pseudomonas putida KT2440 was engineered to metabolize C-polyamide monomers such as 6-aminohexanoic acid, ε-caprolactam and 1,6-hexamethylenediamine, guided by adaptive laboratory evolution. Heterologous expression of nylonases also enabled P. putida to metabolize linear and cyclic nylon oligomers derived from chemical polyamide hydrolysis. RNA sequencing and reverse engineering revealed the metabolic pathways for these non-natural substrates. To demonstrate microbial upcycling, the phaCAB operon from Cupriavidus necator was heterologously expressed to enable production of polyhydroxybutyrate (PHB) from PA6 hydrolysates. This study presents a microbial host for the biological conversion, in combination with chemical hydrolysis, of polyamide monomers and mixed polyamids hydrolysates to a value-added product.

摘要

脂肪族聚酰胺，即尼龙，因其高耐用性和拉伸强度而广泛应用于纺织和汽车行业，但回收率低于5%。聚酰胺的化学回收是可行的，但通常会产生单体和低聚物的混合物，这阻碍了下游的提纯。在此，通过适应性实验室进化，对恶臭假单胞菌KT2440进行工程改造，使其能够代谢C-聚酰胺单体，如6-氨基己酸、ε-己内酰胺和1,6-己二胺。尼龙酶的异源表达也使恶臭假单胞菌能够代谢化学聚酰胺水解产生的线性和环状尼龙低聚物。RNA测序和逆向工程揭示了这些非天然底物的代谢途径。为了证明微生物升级循环利用，来自食酸丛毛单胞菌的phaCAB操纵子被异源表达，从而能够从PA6水解产物中生产聚羟基丁酸酯（PHB）。本研究展示了一种微生物宿主，可将聚酰胺单体和混合聚酰胺水解产物通过生物转化并结合化学水解，转化为高附加值产品。

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