Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Cell Immunol. 2010;264(1):86-92. doi: 10.1016/j.cellimm.2010.05.002. Epub 2010 May 10.
Human NK cells are classified into two populations according to the intensity of CD56 surface expression, as well as possession of CD16, FcRIII. CD56(dim)CD16(bright) make up 90% circulating NK cells, whereas CD56(bright)CD16(-/dim) comprises the remaining 10%. Here we report that peripheral NK cells upon CD16 cross-linking up-regulates the expression of activating markers and receptors such as CD25, CD69, NKp44, NKp30, CD40L and the intensity of CD56 expression. Additionally, co-culturing immature DCs with CD16 activated NK cells was found to significantly increase the expression of maturation markers on DCs. These results suggest that CD16 cross-linking on resting peripheral blood NK cells triggers the activation of these cells and induces the appearance of CD56(bright) NK cells. The latter were found capable of producing pro-inflammatory cytokines, IFN-gamma and TNF-alpha and notably IL-12.
人类 NK 细胞根据 CD56 表面表达强度和 CD16、FcRIII 的存在情况分为两类。CD56(dim)CD16(bright)组成了 90%的循环 NK 细胞，而 CD56(bright)CD16(-/dim)占剩余的 10%。在这里，我们报告称，外周 NK 细胞在 CD16 交联后会上调表达激活标记物和受体，如 CD25、CD69、NKp44、NKp30、CD40L 和 CD56 表达强度。此外，我们发现将未成熟的 DC 与 CD16 激活的 NK 细胞共培养可显著增加 DC 上成熟标志物的表达。这些结果表明，静止外周血 NK 细胞上的 CD16 交联触发这些细胞的激活，并诱导 CD56(bright)NK 细胞的出现。后者被发现能够产生促炎细胞因子 IFN-γ和 TNF-α，特别是 IL-12。
