Scordamaglia Francesca, Balsamo Mirna, Scordamaglia Antonio, Moretta Alessandro, Mingari Maria Cristina, Canonica Giorgio Walter, Moretta Lorenzo, Vitale Massimo
Clinica Malattie dell'Apparato Respiratorio e Allergologia, Dipartimento di Medicina Interna, Università di Genova, Genoa, Italy.
J Allergy Clin Immunol. 2008 Feb;121(2):479-85. doi: 10.1016/j.jaci.2007.09.047. Epub 2007 Dec 3.
Allergic diseases are characterized by abnormal responses to allergens favored by an inappropriate regulation of the T(H)1-T(H)2 polarization. Natural killer (NK) cells give rise to a complex NK/dendritic cell (DC) cross-talk that would help T(H)1 responses.
By analyzing peripheral blood NK cells from 12 patients with either allergic rhinitis or rhinitis and intermittent asthma, we evaluated whether these cells were impaired in their ability to interact with DCs.
Different circulating NK cell subsets were analyzed by flow cytofluorimetry. Mixed NK/DC cultures were performed to assess the reciprocal functional interactions. NK cells were analyzed for their ability to induce DC maturation and cytokine production, and to kill immature DCs. In addition, DCs were assessed for their ability to induce cytokine production by NK cells.
We first analyzed the CD56++CD16+/- cells, a subset of circulating NK cells that is able to respond to DCs by proliferating and producing IFN-gamma. Our analysis revealed that this NK cell subpopulation was significantly reduced in most patients. This was reflected by reduced NK cell-mediated IFN-gamma production in response to DCs. Also, the capability of promoting DC maturation and/or killing immature DCs, a function sustained by CD56+CD16+ NK cells, was reduced in most patients.
We suggest that allergic diseases are accompanied by a partial impairment of the NK cell capability of promoting and maintaining appropriate T(H)1 responses.
过敏性疾病的特征是对过敏原的异常反应，这是由T(H)1 - T(H)2极化的不适当调节所促成的。自然杀伤（NK）细胞会引发复杂的NK/树突状细胞（DC）相互作用，这有助于T(H)1反应。
通过分析12例过敏性鼻炎或鼻炎合并间歇性哮喘患者的外周血NK细胞，我们评估了这些细胞与DC相互作用的能力是否受损。
通过流式细胞荧光术分析不同循环NK细胞亚群。进行NK/DC混合培养以评估相互的功能相互作用。分析NK细胞诱导DC成熟和细胞因子产生以及杀伤未成熟DC的能力。此外，评估DC诱导NK细胞产生细胞因子的能力。
我们首先分析了CD56++CD16+/-细胞，这是循环NK细胞的一个亚群，能够通过增殖和产生IFN-γ对DC作出反应。我们的分析显示，在大多数患者中，这个NK细胞亚群显著减少。这反映在NK细胞对DC反应介导的IFN-γ产生减少。此外，在大多数患者中，由CD56+CD16+ NK细胞维持的促进DC成熟和/或杀伤未成熟DC的能力降低。
我们认为过敏性疾病伴有NK细胞促进和维持适当T(H)1反应能力的部分受损。
