Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Bioresour Technol. 2010 Oct;101(19):7679-82. doi: 10.1016/j.biortech.2010.05.004.
The production of xylooligosaccharides (XOs) from corncobs was studied using a two-stage process based on a steam explosion pretreatment followed by enzymatic hydrolysis. Corncobs with different chip sizes were subjected to steam explosion under different experimental conditions of temperature and time, namely 188-204 degrees C for 2.5-7.5 min. The results indicate that corncobs were optimally steam exploded at 196 degrees C for 5 min, resulting in hemicellulose recovery of 22.8%. Especially, corncobs with large chip sizes (approximately 100 mm) during steam explosion pretreatment were suitable to produce XOs. Furthermore, a thermostable xylanase from Paecilomyces themophila J18 was used to hydrolyze steam explosion liquor of corncobs (SELC) for the production of XOs. A maximum XOs yield of 28.6 g XOs/100 g xylan in corncobs was achieved and XOs syrup contained more than 90% of xylobiose and xylotriose when the hydrolysis was carried out under the optimized conditions (pH 7.0, 70 degrees C, 7.5 U mL(-1) and 2.5 h). These results suggest that the process might be effective in production of XOs for industrial applications.
以蒸汽爆破预处理 followed by 酶解的两段式工艺，研究了从玉米芯生产木低聚糖（XOs）。采用不同温度和时间的实验条件，对不同粒径的玉米芯进行蒸汽爆破，温度分别为 188-204 摄氏度，时间分别为 2.5-7.5 分钟。结果表明，196 摄氏度下爆破 5 分钟可使玉米芯达到最佳爆破效果，半纤维素回收率为 22.8%。特别是在蒸汽爆破预处理时采用大粒径（约 100 毫米）的玉米芯，适合生产 XOs。此外，还使用来自嗜热毁丝霉 J18 的耐热木聚糖酶水解玉米芯蒸汽爆破液（SELC），以生产 XOs。在优化条件下（pH 值 7.0、70 摄氏度、7.5 U mL(-1) 和 2.5 小时），玉米芯中 XOs 的最大产量达到 28.6 g XOs/100 g 木聚糖，XOs 糖浆中含有超过 90%的木二糖和木三糖。这些结果表明，该工艺可能对工业应用中 XOs 的生产有效。
