BRD School of Biosciences, Sardar Patel Maidan, Satellite Campus, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India.
Bioresour Technol. 2012 Jul;115:215-21. doi: 10.1016/j.biortech.2011.10.083. Epub 2011 Oct 31.
The selective production of xylooligosaccharides (XOS) was carried out using partially purified xylanase from Aspergillus foetidus MTCC 4898. Corncob xylan was extracted using a mild alkali treatment which yielded 178.73±5.8 g of xylan/kg of corncobs. Partially purified β-xylosidase free xylanase was found efficient in releasing xylooligosaccharides from corncob xylan. Maximum yield of xylooligosaccharides was 6.73±0.23 mg/ml after 8 h of reaction time using 20 U of xylanase at 45°C. Purification of XOS was done using activated charcoal column chromatography. The purified XOS preparation contained mainly xylobiose and xylotriose. XOS mixture was found suitable for food industry looking at its high thermal stability at low pH. Prebiotic effect of XOS was evaluated by in vitro fermentation of XOS using known probiotic strains viz. Bifidobacterium adolescentis, Bifidobacterium bifidum, Lactobacillus fermentum, Lactobacillus acidophilus. The results of this study revealed better growth of Bifidobacterium spp. on XOS than Lactobacillus spp.
采用黑曲霉 MTCC 4898 部分纯化的木聚糖酶进行木二糖（XOS）的选择性生产。采用温和碱处理从玉米芯中提取木聚糖，得到 178.73±5.8 g 木聚糖/kg 玉米芯。发现部分纯化的无β-木糖苷酶的木聚糖酶能够有效地从玉米芯木聚糖中释放木二糖。在 45°C 下，反应 8 小时，使用 20 U 木聚糖酶，可获得最大的 6.73±0.23 mg/ml 的木二糖产量。采用活性炭柱层析对 XOS 进行纯化。纯化的 XOS 制剂主要含有木二糖和木三糖。从 XOS 在低 pH 值下的高热稳定性来看，XOS 混合物适合食品工业。通过使用已知的益生菌菌株（即双歧杆菌、两歧双歧杆菌、发酵乳杆菌和嗜酸乳杆菌）对 XOS 进行体外发酵，评估 XOS 的益生元作用。这项研究的结果表明，XOS 对双歧杆菌属的生长优于乳杆菌属。
