Characterization of a processed pseudogene derived from the gene encoding bovine adenylate kinase isozyme 2.

We have isolated an intronless processed pseudogene for an isoform of bovine adenylate kinase isozyme 2 (AK2B). The pseudogene which was flanked by 11-nucleotide (nt) direct repeats contained an open reading frame of 519 bp which encodes 173 amino acids (aa), 61 aa shorter than AK2B. In the entire pseudogene sequence, substitutions as well as deletions and insertions were noted in 78 nt positions as compared with the AK2B cDNA (95.1% homology). Northern-blot analysis showed that this pseudogene was not expressed in bovine tissues.