School of Kinesiology and Health Studies, Queen's University, Kingston, ON K7L 3N6, Canada.
Appl Physiol Nutr Metab. 2010 Jun;35(3):350-7. doi: 10.1139/H10-030.
The effects of training on silent mating-type information regulator 2 homolog 1 (SIRT1) activity and protein in relationship to peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) and mitochondrial content were determined in human skeletal muscle. Six weeks of high-intensity interval training ( approximately 1 h of 10 x 4 min intervals at 90% peak oxygen consumption separated by 2 min rest, 3 days per week) increased maximal activities of mitochondrial enzymes in skeletal muscle by 28% to 36% (citrate synthase, beta-hydroxyacyl-coenzyme A dehydrogenase, and cytochrome c oxidase subunit IV) and PGC-1alpha protein (16%) when measured 4 days after training. Interestingly, total muscle SIRT1 activity (31%) and activity per SIRT1 protein (58%) increased despite decreased SIRT1 protein (20%). The present data demonstrate that exercise-induced mitochondrial biogenesis is accompanied by elevated SIRT1 activity in human skeletal muscle.
研究人员在人体骨骼肌中探究了训练对沉默交配型信息调节因子 2 同源物 1（SIRT1）活性和蛋白的影响，以及其与过氧化物酶体增殖物激活受体γ共激活因子 1α（PGC-1α）和线粒体含量的关系。6 周的高强度间歇训练（每周 3 天，约 1 小时，由 10 次 4 分钟间隔组成，间隔休息 2 分钟，峰值耗氧量的 90%）可使骨骼肌中线粒体酶的最大活性增加 28%至 36%（柠檬酸合酶、β-羟酰基辅酶 A 脱氢酶和细胞色素 c 氧化酶亚基 IV）和 PGC-1α 蛋白（16%），在训练后 4 天进行测量时。有趣的是，尽管 SIRT1 蛋白减少了 20%，但总肌肉 SIRT1 活性（31%）和 SIRT1 蛋白活性（58%）却增加了。本研究数据表明，运动引起的线粒体生物发生伴随着人体骨骼肌中 SIRT1 活性的升高。
