Sport and Exercise Sciences Research Institute, Ulster University, Belfast, BT15 1AP, UK.
Personalised Medicine Centre, School of Medicine, Ulster University, Derry/Londonderry, BT47 6SB, UK.
Sci Rep. 2023 Sep 7;13(1):14752. doi: 10.1038/s41598-023-38843-x.
Sirtuin 1 (SIRT1) is a key physiological regulator of metabolism and a target of therapeutic interventions for cardiometabolic and ageing-related disorders. Determining the factors and possible mechanisms of acute and adaptive SIRT1 response to exercise is essential for optimising exercise interventions aligned to the prevention and onset of disease. Exercise-induced SIRT1 upregulation has been reported in animals, but, to date, data in humans have been inconsistent. This exploratory systematic review and meta-analysis aims to assess various exercise interventions measuring SIRT1 in healthy participants. A total of 34 studies were included in the meta-analysis (13 single bout exercise, 21 training interventions). Studies were grouped according to tissue sample type (blood, muscle), biomarkers (gene expression, protein content, enzyme level, enzyme activity), and exercise protocols. A single bout of high-intensity or fasted exercise per se increases skeletal muscle SIRT1 gene expression as measured by qPCR or RT-PCR, while repeated resistance training alone increases blood SIRT1 levels measured by ELISA. A limited number of studies also show a propensity for an increase in muscle SIRT1 activity as measured by fluorometric or sirtuin activity assay. In conclusion, exercise acutely upregulates muscle SIRT1 gene expression and chronically increases SIRT1 blood enzyme levels.
Sirtuin 1（SIRT1）是代谢的关键生理调节剂，也是治疗与心脏代谢和衰老相关疾病的干预靶点。确定急性和适应性 SIRT1 对运动反应的因素和可能机制对于优化与疾病预防和发病相关的运动干预至关重要。运动可诱导动物 SIRT1 的上调，但迄今为止，人类的数据一直不一致。本探索性系统评价和荟萃分析旨在评估各种测量健康参与者 SIRT1 的运动干预。荟萃分析共纳入 34 项研究（13 项单次运动，21 项训练干预）。根据组织样本类型（血液、肌肉）、生物标志物（基因表达、蛋白质含量、酶水平、酶活性）和运动方案对研究进行分组。单次高强度或空腹运动本身就会增加骨骼肌 SIRT1 的基因表达，如 qPCR 或 RT-PCR 所示，而单独进行多次抗阻训练则会增加血液 SIRT1 水平，如 ELISA 所示。少数研究还表明，肌肉 SIRT1 活性（如荧光或 Sirtuin 活性测定）也有增加的趋势。总之，运动可急性上调肌肉 SIRT1 的基因表达，慢性增加 SIRT1 血液酶水平。
