化学绘图与与等能寡核苷酸微阵列的结合比较，揭示了出人意料的枯草芽孢杆菌 RNase P RNA 特异性结构域的结合模式。

Department of Chemistry, University of Rochester, Rochester, New York 14627, USA.

Biochemistry. 2010 Sep 21;49(37):8155-68. doi: 10.1021/bi100286n.

Microarrays with isoenergetic pentamer and hexamer 2'-O-methyl oligonucleotide probes with LNA (locked nucleic acid) and 2,6-diaminopurine substitutions were used to probe the binding sites on the RNase P RNA specificity domain of Bacillus subtilis. Unexpected binding patterns were revealed. Because of their enhanced binding free energies, isoenergetic probes can break short duplexes, merge adjacent loops, and/or induce refolding. This suggests new approaches to the rational design of short oligonucleotide therapeutics but limits the utility of microarrays for providing constraints for RNA structure determination. The microarray results are compared to results from chemical mapping experiments, which do provide constraints. Results from both types of experiments indicate that the RNase P RNA folds similarly in 1 M Na(+) and 10 mM Mg(2+).

使用具有 LNA（锁核酸）和 2,6-二氨基嘌呤取代的等能量五聚体和六聚体 2'-O-甲基寡核苷酸探针的微阵列来探测枯草芽孢杆菌 RNase P RNA 特异性结构域上的结合位点。揭示了意想不到的结合模式。由于它们增强的结合自由能，等能量探针可以打破短双链体，合并相邻环，和/或诱导重折叠。这为合理设计短寡核苷酸治疗药物提供了新的方法，但限制了微阵列在提供 RNA 结构确定约束方面的效用。将微阵列结果与确实提供约束的化学作图实验结果进行比较。两种类型的实验结果均表明，RNase P RNA 在 1 M Na(+) 和 10 mM Mg(2+)中折叠相似。