Division of Hepatobiliary and Pancreatic Diseases, Department of Internal Medicine, Hyogo College of Medicine, Tokyo, Japan.
Hepatol Res. 2010 Aug;40(8):823-31. doi: 10.1111/j.1872-034X.2010.00670.x. Epub 2010 Jun 8.
To evaluate the dynamics of Kupffer cell (KC) phagocytosis by performing both in vivo and in vitro studies using Sonazoid (GE Healthcare, Oslo) in a rat nonalcoholic steatohepatitis (NASH) model.
Contrast enhanced ultrasonography (CEUS) was performed on a rat NASH model induced by a methionine choline deficient diet (MCDD) and control rats, and Sonazoid was used to measure the signal intensity in the liver parenchyma. The uptake of Sonazoid by the KCs was observed by intravital microscopy. Their phagocytic capability was evaluated in vitro using isolated and cultured KCs. The uptake of fluorescein isothiocyanate (FITC)-labeled latex beads was observed and quantitatively analyzed by flow cytometry.
In the MCDD group, liver parenchymal enhancement was reduced 20 min after the Sonazoid injection. Microscopic observation of the isolated and cultured KCs revealed that the number of phagocytosed Sonazoid microbubbles was significantly decreased. Confocal laser scanning microscopic (CLSM) observation showed a decrease in the uptake of the latex beads. A decreased phagocytic capacity in the MCDD group was suggested by the quantitative analysis using flow cytometry, as well as by intravital microscopy.
CEUS with Sonazoid is a powerful evaluation tool to diagnose NASH from an early stage of the disease.
通过使用 SonoVue（GE Healthcare，奥斯陆）在大鼠非酒精性脂肪性肝炎（NASH）模型中进行体内和体外研究，评估枯否细胞（KC）吞噬作用的动力学。
对采用蛋氨酸胆碱缺乏饮食（MCDD）诱导的大鼠 NASH 模型和对照大鼠进行对比增强超声检查（CEUS），并使用 SonoVue 测量肝实质中的信号强度。通过活体显微镜观察 SonoVue 被 KC 摄取的情况。使用分离和培养的 KC 评估其体外吞噬能力。通过流式细胞术观察并定量分析异硫氰酸荧光素（FITC）标记的乳胶珠的摄取情况。
在 MCDD 组中，Sonazoid 注射后 20 分钟肝实质增强减少。对分离和培养的 KC 的显微镜观察表明，吞噬的 SonoVue 微泡数量明显减少。共聚焦激光扫描显微镜（CLSM）观察显示乳胶珠的摄取减少。通过流式细胞术的定量分析以及活体显微镜观察，提示 MCDD 组的吞噬能力降低。
SonoVue 的 CEUS 是从疾病早期诊断 NASH 的有力评估工具。
