Watanabe Rira, Matsumura Manabu, Munemasa Toshiko, Fujimaki Masayoshi, Suematsu Makoto
R&D Division, Daiichi Pharmaceutical Co., Ltd, Tokyo, Japan.
Invest Radiol. 2007 Sep;42(9):643-51. doi: 10.1097/RLI.0b013e31805f2682.
The objective of this study was to elucidate the mechanism of hepatic parenchyma-specific contrast of Sonazoid (microbubble contrast agent) using microscopic techniques.
Sonazoid was intravenously injected into rats to investigate the microbubble dynamics and distribution within hepatic microcirculation in exteriorized liver using intravital microscopy and to observe dose dependency of ultrasound hepatic contrast effect. In vitro and in vivo uptake of microbubbles by Kupffer cells was examined using confocal laser scanning microscopy.
Intravital observation demonstrated freely flowing microbubbles in the sinusoid and some microbubbles co-localized with Kupffer cells. The microbubbles internalized in Kupffer cells were identified with reflected light by confocal laser scanning microscopy. The percentage of Kupffer cells taking up microbubbles was about 1% at clinical dose at which the homogeneous hepatic contrast was observed.
The hepatic parenchyma-specific contrast by Sonazoid is due to distribution of the microbubbles in Kupffer cells.
本研究的目的是利用显微技术阐明声诺维（微泡造影剂）肝实质特异性显影的机制。
将声诺维静脉注射到大鼠体内，采用活体显微镜观察肝微循环中微泡的动力学和分布情况，并观察超声肝脏造影效果的剂量依赖性。使用共聚焦激光扫描显微镜检查库普弗细胞对微泡的体外和体内摄取情况。
活体观察显示，微泡在肝血窦中自由流动，部分微泡与库普弗细胞共定位。共聚焦激光扫描显微镜通过反射光识别出被库普弗细胞摄取的微泡。在观察到肝脏均匀显影的临床剂量下，摄取微泡的库普弗细胞百分比约为1%。
声诺维的肝实质特异性显影是由于微泡在库普弗细胞中的分布所致。
