Brick-Ghannam C, Mooney N, Charron D
Laboratoire d'Immunogénétique Moléculaire, Institut des Cordeliers, Paris, France.
Hum Immunol. 1991 Mar;30(3):202-7. doi: 10.1016/0198-8859(91)90035-8.
We have examined the activity and intracellular compartmentalization of protein kinase C (PKC) following activation of human B lymphocytes by anti-human leukocyte antigen (HLA) class II antibodies. 12-O-Tetradecanoylphorbol 13-acetate (TPA) treatment increased membrane-associated PKC (between five and nine times greater than the control value) and decreased cytosolic PKC (between 70% and 100% of the control value). In contrast, anti-class II antibodies induce an activation of PKC which results either in an increase of cytosolic activity or membrane-bound activity without redistribution of cytosolic PKC. The effect of TPA and HLA class II molecules on total PKC activity was comparable: when TPA induced an increase of total PKC activity so did HLA class II molecules and when TPA did not, HLA class II molecules did not. Measurement on SDS PAGE of histone phosphorylation confirmed the above results of PKC activity. Taken together, our results suggest that PKC might be implicated in HLA class II-induced B lymphocyte activation.
我们已经检测了抗人白细胞抗原（HLA）II类抗体激活人B淋巴细胞后蛋白激酶C（PKC）的活性及细胞内定位。12 - 十四烷酰佛波醇-13 - 乙酸酯（TPA）处理可增加膜相关PKC（比对照值高5至9倍）并降低胞质PKC（为对照值的70%至100%）。相比之下，抗II类抗体诱导PKC激活，其结果要么是胞质活性增加，要么是膜结合活性增加，而胞质PKC无重新分布。TPA和HLA II类分子对总PKC活性的影响相当：当TPA诱导总PKC活性增加时，HLA II类分子也会如此；当TPA不诱导时，HLA II类分子也不会。在SDS - PAGE上对组蛋白磷酸化的测定证实了上述PKC活性结果。综上所述，我们的结果表明PKC可能参与了HLA II类分子诱导的B淋巴细胞激活。
