Brick-Ghannam C, Charron D
Laboratoire d'immunogénétique moléculaire, Institut des Cordeliers, Paris, France.
Bull Cancer. 1993 Sep;80(9):780-5.
HLA class II antigens are involved in signal transduction in B lymphocytes. We have previously reported that the ligation of HLA class II antigens in B cells results in an increase of cytosolic and membrane PKC activity [3]. Unlike TPA, no translocation of the cytosolic PKC was observed following anti HLA class II antibody treatment. However, an increase of both PKC activity (cytosolic and membrane) and quantity was observed. These effects are completely abolished by actinomycin D treatment. Northern blot analysis and PCR reaction revealed that anti HLA class II antibodies induce an increase of the PKC beta level which is significant after 20 minutes of stimulation and rose to a maximum after 60 minutes. Taken together, our results show that anti HLA class II antibodies exert a transcriptional regulation of PKC in human B lymphocytes.
HLA II类抗原参与B淋巴细胞的信号转导。我们之前报道过，B细胞中HLA II类抗原的连接会导致胞质和膜PKC活性增加[3]。与佛波酯不同，抗HLA II类抗体处理后未观察到胞质PKC的转位。然而，观察到PKC活性（胞质和膜）及数量均增加。放线菌素D处理可完全消除这些效应。Northern印迹分析和PCR反应显示，抗HLA II类抗体诱导PKCβ水平升高，刺激20分钟后显著升高，60分钟后升至最高。综上所述，我们的结果表明抗HLA II类抗体对人B淋巴细胞中的PKC发挥转录调控作用。
