Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7 St., 30-387 Krakow, Poland.
Dev Comp Immunol. 2010 Oct;34(10):1129-36. doi: 10.1016/j.dci.2010.06.005. Epub 2010 Jun 23.
To date, functioning of insect humoral immune response is especially well described in Diptera. The mechanisms of pathogen recognition, activation of signaling pathways and regulation of antimicrobial defense peptide expression are relatively well known. The present paper demonstrates evidence that the immune system of the Lepidoptera moth, Galleria mellonella, is also able to distinguish between different classes of microorganisms and responds to the invading pathogen accordingly. G. mellonella larvae were challenged with Gram-negative and Gram-positive bacteria as well as with yeast and filamentous fungus cells. Subsequently, 24, 48 and 72 h after immunization, the concentrations of lysozyme and six defense peptides were determined in the hemolymph by the HPLC technique. The compounds studied demonstrated variability both in the kinetics of the increase as well as in the concentrations reached. The Gram-negative bacterium and filamentous fungus were particularly effective immunogens, especially affecting the levels of lysozyme, Galleria defensin, proline-rich peptide 2 and cecropin D-like peptide.
迄今为止，昆虫体液免疫反应的功能在双翅目昆虫中得到了特别详细的描述。病原体识别、信号通路激活和抗菌防御肽表达的调控机制已被广泛了解。本文证明了鳞翅目蛾类，欧洲山黑蜂幼虫的免疫系统也能够区分不同类别的微生物，并相应地对入侵的病原体作出反应。用革兰氏阴性和革兰氏阳性细菌以及酵母和丝状真菌细胞对欧洲山黑蜂幼虫进行了挑战。随后，在免疫后 24、48 和 72 小时，通过 HPLC 技术测定了血淋巴中的溶菌酶和六种防御肽的浓度。研究的化合物在增加的动力学和达到的浓度方面都表现出了可变性。革兰氏阴性菌和丝状真菌是特别有效的免疫原，特别是对溶菌酶、防御肽、富含脯氨酸的肽 2 和抗菌肽 D 样肽的水平有影响。
