Department of Physiology (Animal Physiology II), Faculty of Biological Sciences, Madrid Complutense University, Spain.
J Gerontol A Biol Sci Med Sci. 2010 Sep;65(9):941-50. doi: 10.1093/gerona/glq101. Epub 2010 Jun 17.
In order to verify the survival biomarker role of several immune functions, and to determine the oxidation and inflammation mechanisms underlying variability in the aging process, we have investigated a variety of immune functions and oxidative stress parameters as well as activation of the nuclear factor kappa B (NF kappaB) in peritoneal leukocytes from four different age groups of mice, including natural extreme longevity. Immune cells from naturally long-lived animals showed preservation of immune function in response to stimuli and controlled oxidative stress as well as nuclear factor kappa B activation in resting conditions. Moreover, leukocytes from extreme long-lived animals showed increased catalase activity when compared with the adults. In contrast, the old and very old animal groups showed impaired immune function and increased oxidation as well as NF kappaB activation. Our results support preserved immune function as a biomarker of extended survival and point to controlled regulation of NF kappaB activity as a key mechanism restraining oxidative stress in immune cells and contributing to reach longevity.
为了验证几种免疫功能的生存生物标志物作用，并确定衰老过程中变异性的氧化和炎症机制，我们研究了来自四个不同年龄组（包括自然极端长寿）的小鼠腹膜白细胞中的多种免疫功能和氧化应激参数以及核因子 kappa B（NF kappaB）的激活。自然长寿动物的免疫细胞在受到刺激时表现出免疫功能的保存，并在静息状态下控制氧化应激和核因子 kappaB 的激活。此外，与成年动物相比，极端长寿动物的白细胞表现出更高的过氧化氢酶活性。相比之下，老年和非常老年动物组表现出免疫功能受损、氧化增加以及 NF kappaB 激活。我们的结果支持免疫功能的保存作为延长生存的生物标志物，并指出 NF kappaB 活性的控制调节是限制免疫细胞中氧化应激并有助于达到长寿的关键机制。
