Department of Genetics, Physiology and Microbiology (Animal Physiology Unit), School of Biology, Complutense University of Madrid (UCM), 28040 Madrid, Spain.
Institute of Investigation of Hospital 12 de Octubre (i+12), 28041 Madrid, Spain.
Int J Mol Sci. 2019 Feb 12;20(3):769. doi: 10.3390/ijms20030769.
Oxidative and inflammatory stresses are closely related processes, which contribute to age-associated impairments that affect the regulatory systems such as the immune system and its immunosenescence. Therefore, the aim of this work was to confirm whether an oxidative/inflammatory stress occurs in immune cells from adult mice with premature aging, similar to that shown in leukocytes from chronologically old animals, and if this results in immunosenescence. Several oxidants/antioxidants and inflammatory/anti-inflammatory cytokines were analyzed in peritoneal leukocytes from adult female CD1 mice in two models of premature aging-(a) prematurely aging mice (PAM) and (b) mice with the deletion of a single allele (hemi-zygotic: HZ) of the tyrosine hydroxylase () gene (TH-HZ), together with cells from chronologically old animals. Several immune function parameters were also studied in peritoneal phagocytes and lymphocytes. The same oxidants and antioxidants were also analyzed in spleen and thymus leukocytes. The results showed that the immune cells of PAM and TH-HZ mice presented lower values of antioxidant defenses and higher values of oxidants/pro-inflammatory cytokines than cells from corresponding controls, and similar to those in cells from old animals. Moreover, premature immunosenescence in peritoneal leukocytes from both PAM and TH-HZ mice was also observed. In conclusion, adult PAM and TH-HZ mice showed oxidative stress in their immune cells, which would explain their immunosenescence.
氧化应激和炎症应激是密切相关的过程，它们共同导致与年龄相关的损伤，影响免疫系统等调节系统及其免疫衰老。因此，本工作的目的是确认是否在具有早衰的成年小鼠的免疫细胞中发生氧化/炎症应激，类似于在来自同期老年动物的白细胞中观察到的情况，以及这是否导致免疫衰老。在两种早衰模型中（a）早衰小鼠（PAM）和（b）酪氨酸羟化酶（）基因单等位基因缺失的小鼠（半合子：HZ）的成年雌性 CD1 小鼠的腹腔白细胞中分析了几种氧化剂/抗氧化剂和炎症/抗炎细胞因子，以及来自同期老年动物的细胞。还研究了腹腔吞噬细胞和淋巴细胞中的几种免疫功能参数。在脾和胸腺白细胞中也分析了相同的氧化剂和抗氧化剂。结果表明，与相应对照细胞相比，PAM 和 TH-HZ 小鼠的免疫细胞的抗氧化防御能力较低，氧化剂/促炎细胞因子的水平较高，与老年动物的细胞相似。此外，还观察到来自 PAM 和 TH-HZ 小鼠的腹腔白细胞的过早免疫衰老。总之，成年 PAM 和 TH-HZ 小鼠的免疫细胞表现出氧化应激，这可以解释它们的免疫衰老。
