Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain.
Institute of Biomedical Research Hospital 12 Octubre (imas12), 28041 Madrid, Spain.
Int J Mol Sci. 2019 Feb 12;20(3):771. doi: 10.3390/ijms20030771.
In Parkinson's Disease (PD), the peripheral changes in the functional capacity and redox state of immune cells has been scarcely investigated, especially in the early PD stages. Aging is a risk factor for PD, and the age-related impairment of the immune system, based on a chronic-oxidative stress situation, is involved in the rate of aging. We analyzed several functions in isolated peripheral blood neutrophils and mononuclear cells from PD stage 2 patients, and compared the results to those in healthy elderly and adult controls. Several oxidative stress and damage parameters were studied in whole blood cells. The results showed an impairment of the lymphoproliferative response in stimulated conditions in the PD patients compared with age-matched controls, who also showed typical immunosenescence in comparison with adult individuals. Higher oxidative stress and damage were observed in whole blood cells from PD patients (lower glutathione peroxidase activity, and higher oxidized glutathione and malondialdehyde contents). Our results suggest an accelerated immunosenescence in PD stage 2, and that several of the parameters studied could be appropriate peripheral biomarkers in the early stages of PD.
在帕金森病（PD）中，免疫细胞的功能和氧化还原状态的外周变化尚未得到充分研究，特别是在早期 PD 阶段。衰老也是 PD 的一个风险因素，基于慢性氧化应激情况的免疫系统的年龄相关性损伤与衰老速度有关。我们分析了来自 PD 2 期患者的分离外周血中性粒细胞和单核细胞的几种功能，并将结果与健康老年人和成人对照组进行了比较。我们还研究了全血细胞中的几种氧化应激和损伤参数。结果显示，与年龄匹配的对照组相比，PD 患者在刺激条件下的淋巴细胞增殖反应受损，而与成人个体相比，对照组也表现出典型的免疫衰老。PD 患者的全血细胞中观察到更高的氧化应激和损伤（谷胱甘肽过氧化物酶活性降低，氧化谷胱甘肽和丙二醛含量升高）。我们的结果表明，PD 2 期存在加速的免疫衰老，并且研究的一些参数可能是 PD 早期的合适的外周生物标志物。
