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严重前阴道壁脱垂女性阴道壁的单细胞转录组分析。

Single-cell transcriptome profiling of the vaginal wall in women with severe anterior vaginal prolapse.

机构信息

Medical Science Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, 100730, Beijing, China.

CAS Key Laboratory of Genomic and Precision Medicine, Collaborative Innovation Center of Genetics and Development, College of Future Technology, Beijing Institute of Genomics, Chinese Academy of Sciences, 100101, Beijing, China.

出版信息

Nat Commun. 2021 Jan 4;12(1):87. doi: 10.1038/s41467-020-20358-y.

DOI:10.1038/s41467-020-20358-y
PMID:33397933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7782707/
Abstract

Anterior vaginal prolapse (AVP) is the most common form of pelvic organ prolapse (POP) and has deleterious effects on women's health. Despite recent advances in AVP diagnosis and treatment, a cell atlas of the vaginal wall in AVP has not been constructed. Here, we employ single-cell RNA-seq to construct a transcriptomic atlas of 81,026 individual cells in the vaginal wall from AVP and control samples and identify 11 cell types. We reveal aberrant gene expression in diverse cell types in AVP. Extracellular matrix (ECM) dysregulation and immune reactions involvement are identified in both non-immune and immune cell types. In addition, we find that several transcription factors associated with ECM and immune regulation are activated in AVP. Furthermore, we reveal dysregulated cell-cell communication patterns in AVP. Taken together, this work provides a valuable resource for deciphering the cellular heterogeneity and the molecular mechanisms underlying severe AVP.

摘要

前阴道脱垂（AVP）是最常见的盆腔器官脱垂（POP）形式，对女性健康有不良影响。尽管最近在 AVP 的诊断和治疗方面取得了进展，但尚未构建 AVP 阴道壁的细胞图谱。在这里，我们采用单细胞 RNA-seq 技术，构建了来自 AVP 和对照样本的 81026 个单个阴道壁细胞的转录组图谱，并鉴定出 11 种细胞类型。我们揭示了 AVP 中不同细胞类型的异常基因表达。在非免疫和免疫细胞类型中均发现细胞外基质（ECM）失调和免疫反应参与。此外，我们发现与 ECM 和免疫调节相关的几个转录因子在 AVP 中被激活。此外，我们揭示了 AVP 中失调的细胞间通讯模式。总之，这项工作为解析严重 AVP 的细胞异质性和分子机制提供了有价值的资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/46c1785c24f4/41467_2020_20358_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/010853a01f14/41467_2020_20358_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/56c16b89e80d/41467_2020_20358_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/542d4fcd07ec/41467_2020_20358_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/d4b9575b47ae/41467_2020_20358_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/46c1785c24f4/41467_2020_20358_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/010853a01f14/41467_2020_20358_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/56c16b89e80d/41467_2020_20358_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/542d4fcd07ec/41467_2020_20358_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/d4b9575b47ae/41467_2020_20358_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4c4/7782707/46c1785c24f4/41467_2020_20358_Fig5_HTML.jpg

相似文献

[1]
Single-cell transcriptome profiling of the vaginal wall in women with severe anterior vaginal prolapse.

Nat Commun. 2021-1-4

[2]
Differential gene expression of extracellular-matrix-related proteins in the vaginal apical compartment of women with pelvic organ prolapse.

Int Urogynecol J. 2019-3

[3]
[Expression and significance of elastin and fibulin-5 in anterior vaginal tissue of women with pelvic organ prolapse].

Zhonghua Fu Chan Ke Za Zhi. 2009-7

[4]
Vaginal smooth muscle cell apoptosis is increased in women with pelvic organ prolapse.

Int Urogynecol J Pelvic Floor Dysfunct. 2008-11

[5]
Changes in tissue composition of the vaginal wall of premenopausal women with prolapse.

Am J Obstet Gynecol. 2013-11-1

[6]
Anatomical outcomes 1 year after pelvic organ prolapse surgery in patients with and without a uterus at a high risk of recurrence: a randomised controlled trial comparing laparoscopic sacrocolpopexy/cervicopexy and anterior vaginal mesh.

Int Urogynecol J. 2019-4

[7]
Expression of extracellular matrix-remodeling proteins is altered in vaginal tissue of premenopausal women with severe pelvic organ prolapse.

Reprod Sci. 2013-12-16

[8]
scRNA-seq reveals aging-related immune cell types and regulators in vaginal wall from elderly women with pelvic organ prolapse.

Front Immunol. 2023

[9]
Local oestrogen therapy modulates extracellular matrix and immune response in the vaginal tissue of post-menopausal women with severe pelvic organ prolapse.

J Cell Mol Med. 2019-2-17

[10]
Comparative Characterization of Vaginal Cells Derived From Premenopausal Women With and Without Severe Pelvic Organ Prolapse.

Reprod Sci. 2016-7

引用本文的文献

[1]
17β-estradiol facilitates the proliferation, migration, and collagen production in uterosacral ligament fibroblasts from patients with pelvic organ prolapse by activating HOXA13/TIMP1 axis.

J Mol Histol. 2025-8-19

[2]
Unveiling pathogenesis of pelvic organ prolapse through transcriptomic and bioinformatic analyses in uterosacral ligament tissues of postmenopausal women.

Front Genet. 2025-6-6

[3]
Epidemiology, etiology and treatment of female vaginal injury.

Reprod Health. 2025-5-6

[4]
Anatomo-pathological aspects of pelvic organ prolapse: analytical study of round and utero-sacral ligaments of Congolese women during the mass campaign in two hospitals of city of Kananga.

Pan Afr Med J. 2025-1-14

[5]
Development and characterization of a novel immortalized human vaginal fibroblast cell line for advanced applications in reproductive health.

Reprod Biol Endocrinol. 2025-4-10

[6]
Recombinant Humanized Collagen: A Promising Treatment for Pelvic Organ Prolapse via Enhanced Fibroblast Function and Angiogenesis.

Int Urogynecol J. 2025-4

[7]
Identification of potential drug targets for pelvic organ prolapse using a proteome-wide Mendelian randomization approach.

Sci Rep. 2025-3-10

[8]
Combining pelvic floor ultrasonography with deep learning to diagnose anterior compartment organ prolapse.

Quant Imaging Med Surg. 2025-2-1

[9]
SIRT1 Alleviates Oxidative Stress-Induced Mitochondrial Dysfunction and Mitochondria-Associated Membrane Dysregulation in Stress Urinary Incontinence.

Cell Prolif. 2025-5

[10]
Comprehensive analysis of the circRNA expression profile and circRNA-miRNA-mRNA network in pelvic organ prolapse.

Front Genet. 2025-1-20

本文引用的文献

[1]
Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging.

Cell. 2020-2-27

[2]
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Cell Mol Life Sci. 2020-8

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Int Braz J Urol. 2020

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Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma.

Cell. 2019-10-31

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Cell. 2019-6-6

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Neurovascular alterations of muscularis propria in the human anterior vaginal wall in pelvic organ prolapse.

J Anat. 2019-5-30

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Cell Syst. 2019-4-3

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Zhonghua Yi Xue Za Zhi. 2019-3-19

[10]
Local oestrogen therapy modulates extracellular matrix and immune response in the vaginal tissue of post-menopausal women with severe pelvic organ prolapse.

J Cell Mol Med. 2019-2-17

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