Damaj M I, Trouvin J H, Lambrey B, Jacquot C
Laboratoire de Pharmacologie et de Pharmacocinétique, Faculté de Pharmacie, Chatenay-Malabry, France.
J Chromatogr. 1991 Feb 15;563(2):476-9. doi: 10.1016/0378-4347(91)80059-l.
A reversed-phase (CN as stationary phase) liquid chromatographic method with electrochemical detection is described for the quantitation of COR 3224, a new 2-amino-2-oxazoline in plasma and brain tissue of the rat. Extraction was performed with dichloromethane and detection was achieved at a working electrode potential of +0.85 V versus an Ag/AgCl reference electrode. The recovery of the method is about 80 and 60% for plasma and brain, respectively. The limit of detection was less than 10 ng/ml for both plasma and brain, five times lower than that with ultraviolet detection.
本文描述了一种采用反相（以CN为固定相）液相色谱法并结合电化学检测的方法，用于定量测定大鼠血浆和脑组织中一种新型2-氨基-2-恶唑啉COR 3224。用二氯甲烷进行萃取，在相对于Ag/AgCl参比电极的工作电极电位为+0.85 V时进行检测。该方法对血浆和脑的回收率分别约为80%和60%。血浆和脑的检测限均小于10 ng/ml，比紫外检测法低五倍。
