Chau H W, Goh Y K, Si B C, Vujanovic Vladimir
Department of Soil Science, University of Saskatchewan, Saskatoon, Canada.
Biotech Histochem. 2011 Aug;86(4):280-7. doi: 10.3109/10520295.2010.492733. Epub 2010 Jun 21.
Most natural and synthetic dyes currently used for microbial fluorescent staining are toxic or carcinogenic and are harmful to animals, humans and the environment. A food dye for microbial staining, brilliant blue FCF, was used as an alternative to lactofuchsin and lactophenol blue. Brilliant blue FCF shows pronounced microbial cell fluorescence staining of an array of pathogenic/toxigenic (Fusarium granunearum 3- and 15-acetyldeoxynivalenol chemotypes, and Escherichia coli O157:H7) and beneficial fungi and bacteria (Trichoderma harzianum and Bacillus subtilis). Brilliant blue FCF has no toxic effects on the microbes tested and is inexpensive.
目前用于微生物荧光染色的大多数天然和合成染料都有毒或致癌，对动物、人类和环境有害。一种用于微生物染色的食用染料亮蓝FCF被用作品红和乳酚蓝的替代品。亮蓝FCF对一系列致病/产毒微生物（禾谷镰刀菌3-和15-乙酰脱氧雪腐镰刀菌烯醇化学型，以及大肠杆菌O157:H7）和有益真菌及细菌（哈茨木霉和枯草芽孢杆菌）表现出明显的微生物细胞荧光染色效果。亮蓝FCF对所测试的微生物没有毒性作用且价格低廉。
