Takahashi K, Naito M, Katabuchi H, Higashi K
Second Department of Pathology, Kumamoto University Medical School, Japan.
J Leukoc Biol. 1991 Jul;50(1):57-68. doi: 10.1002/jlb.50.1.57.
In blood vessels of the chorionic villi of mouse placenta, primitive macrophages first emerged at 10 days of gestation, then differentiated and matured into fetal macrophages. After emigration into the chorionic villous mesenchymal stroma, they ingested fluid-like stromal materials and transformed into Hofbauer cells. In our observation of their differentiation and maturation, no promonocytes or monocytes were detected. In the culture of cell suspensions from the placenta with LP3-conditioned medium, CFU-GM was confirmed, but in the culture with the mouse bone marrow stromal cell line (ST2) the primitive/fetal macrophage population occurred predominantly before the development of the monocyte/macrophage population. Proliferative potential of the primitive and fetal macrophages is slight. With the progress of gestation, the monocyte/macrophage population appeared in the chorionic villous stroma, forming a heterogeneous population of placental macrophages in the late fetal stage.
在小鼠胎盘绒毛膜绒毛的血管中，原始巨噬细胞最早在妊娠10天时出现，随后分化并成熟为胎儿巨噬细胞。迁移到绒毛膜绒毛间充质基质后，它们摄取类液体基质物质并转化为霍夫鲍尔细胞。在我们对其分化和成熟的观察中，未检测到前单核细胞或单核细胞。在用LP3条件培养基培养胎盘细胞悬液时，证实了CFU-GM的存在，但在用小鼠骨髓基质细胞系（ST2）培养时，原始/胎儿巨噬细胞群体主要在单核细胞/巨噬细胞群体发育之前出现。原始和胎儿巨噬细胞的增殖潜力较小。随着妊娠的进展，单核细胞/巨噬细胞群体出现在绒毛膜绒毛基质中，在胎儿后期形成胎盘巨噬细胞的异质群体。
