Yamabe S
J Gen Microbiol. 1978 Apr;105(2):227-32. doi: 10.1099/00221287-105-2-227.
Reduction of cytochrome c from both horse heart and Candida krusei by FeSO4 has been demonstrated. This reaction was stimulated by nalidixic acid and structurally related compounds, and the effect was more pronounced for the yeast cytochrome. Divalent metal ions other than Fe2+ lessened or abolished the stimulation by these compounds. Fe2+ and other metal ions altered the spectra of nalidixic acid and related compounds indicating the formation of metal chelate complexes. 1,10-Phenanthroline inhibited reduction of cytochrome c b Fe2+. Other divalent metal ions relieved the inhibition, probably by forming chelates with 1,10-phenanthroline. The results suggest that metal ion chelation may be involved in the molecular mode of action of nalidixic acid and related drugs. The relevance of this artificial electron transfer system to bacterial electron transfer in vivo is discussed.
已证明硫酸亚铁可使马心和克鲁斯假丝酵母中的细胞色素c还原。该反应受到萘啶酸及结构相关化合物的刺激，且对酵母细胞色素的作用更为明显。除Fe2+外的二价金属离子会减弱或消除这些化合物的刺激作用。Fe2+和其他金属离子改变了萘啶酸及相关化合物的光谱，表明形成了金属螯合物。1,10-菲咯啉抑制细胞色素c被Fe2+还原。其他二价金属离子可能通过与1,10-菲咯啉形成螯合物来解除这种抑制。结果表明，金属离子螯合可能参与了萘啶酸及相关药物的分子作用模式。文中还讨论了这种人工电子传递系统与体内细菌电子传递的相关性。
