Luukkonen Jukka, Juutilainen Jukka, Naarala Jonne
Department of Environmental Science, University of Eastern Finland, Kuopio, Finland.
Bioelectromagnetics. 2010 Sep;31(6):417-24. doi: 10.1002/bem.20580.
The aim of the present study was to investigate possible cooperative effects of radiofrequency (RF) radiation and ferrous chloride (FeCl(2)) on reactive oxygen species (ROS) production and DNA damage. In order to test intracellular ROS production as a possible underlying mechanism of DNA damage, we applied the fluorescent probe DCFH-DA. Integrity of DNA was quantified by alkaline comet assay. The exposures to 872 MHz RF radiation were conducted at a specific absorption rate (SAR) of 5 W/kg using continuous waves (CW) or a modulated signal similar to that used in Global System for Mobile Communications (GSM) phones. Four groups were included: (1) Sham exposure (control), (2) RF radiation, (3) Chemical treatment, (4) Chemical treatment, and RF radiation. In the ROS production experiments, human neuroblastoma (SH-SY5Y) cells were exposed to RF radiation and 10 microg/ml FeCl(2) for 1 h. In the comet assay experiments, the exposure time was 3 h and an additional chemical (0.015% diethyl maleate) was used to make DNA damage level observable. The chemical treatments resulted in statistically significant responses, but no effects from either CW or modulated RF radiation were observed on ROS production, DNA damage or cell viability.
本研究的目的是调查射频（RF）辐射与氯化亚铁（FeCl₂）对活性氧（ROS）生成及DNA损伤可能产生的协同作用。为了检测细胞内ROS生成作为DNA损伤潜在机制的可能性，我们应用了荧光探针2',7'-二氯二氢荧光素二乙酸酯（DCFH-DA）。通过碱性彗星试验对DNA完整性进行定量分析。使用连续波（CW）或类似于全球移动通信系统（GSM）手机所使用的调制信号，以5 W/kg的比吸收率（SAR）进行872 MHz RF辐射暴露实验。实验分为四组：（1）假暴露（对照组），（2）RF辐射组，（3）化学处理组，（4）化学处理与RF辐射组。在ROS生成实验中，人神经母细胞瘤（SH-SY5Y）细胞暴露于RF辐射及10 μg/ml FeCl₂ 中1小时。在彗星试验实验中，暴露时间为3小时，并使用另一种化学物质（0.015%马来酸二乙酯）以使DNA损伤水平可观察到。化学处理产生了具有统计学意义的反应，但未观察到CW或调制RF辐射对ROS生成、DNA损伤或细胞活力有任何影响。
