INRA-Laboratoire de Nutrition et Régulations Lipidiques des Fonctions Cérébrales, UR909, F-78352 Jouy en Josas, France.
J Cell Biochem. 2010 Aug 15;110(6):1356-64. doi: 10.1002/jcb.22652.
Rat neural stem cells/neural progenitors (NSC/NP) are generally grown in serum-free medium. In this study, NSC/NP were supplemented with the main long-chain polyunsaturated fatty acids (PUFAs) present in the brain, arachidonic acid (AA), or docosahexaenoic acid (DHA), and were monitored for their growth. Lipid and fatty acid contents of the cells were also determined. Under standard conditions, the cells were characterized by phospholipids displaying a highly saturated profile, and very low levels of PUFAs. When cultured in the presence of PUFAs, the cells easily incorporated them into the phospholipid fraction. We also compared the presence of three membrane proteins in the lipid raft fractions: GFR and connexin 43 contents in the rafts were increased by DHA supplementation, whereas Gbeta subunit content was not significantly modified. The restoration of DHA levels in the phospholipids could profoundly affect protein localization and, consequently, their functionalities.
大鼠神经干细胞/神经前体细胞（NSC/NP）通常在无血清培养基中培养。在本研究中，向 NSC/NP 中添加大脑中存在的主要长链多不饱和脂肪酸（PUFA），即花生四烯酸（AA）或二十二碳六烯酸（DHA），并监测其生长情况。还测定了细胞的脂质和脂肪酸含量。在标准条件下，细胞的特征是磷脂显示出高度饱和的图谱，以及极低水平的 PUFAs。当在 PUFAs 的存在下培养时，细胞很容易将它们掺入磷脂部分。我们还比较了脂质筏部分中三种膜蛋白的存在情况：DHA 补充增加了 GFR 和连接蛋白 43 在筏中的含量，而 Gβ亚基含量没有明显改变。磷脂中 DHA 水平的恢复可能会深刻影响蛋白质的定位，从而影响它们的功能。
