Institute of Physiological Chemistry, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany.
Cell Immunol. 2012 Jan-Feb;275(1-2):42-6. doi: 10.1016/j.cellimm.2012.03.004. Epub 2012 Mar 27.
In the present study, the lipid raft composition of a canine mastocytoma cell line (C2) was analyzed. Lipid rafts were well separated from non-raft plasma membranes using a detergent-free isolation technique. To study the influence of n-3 and n-6 polyunsaturated fatty acids (PUFA) on raft fatty acid composition in comparison to non-raft cell membrane, C2 were supplemented with one of the following: α-linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, linoleic acid or arachidonic acid. Enrichment of the culture medium with a specific PUFA resulted in an increase in the content of this fatty acid both in rafts and non-raft membranes. Contents of cholesterol and protein were found not to be affected by the changes in the fatty acid profiles. In conclusion, our data provide strong evidence that PUFA modulate lipid composition and physiological properties of membrane micro domains of mast cells which in turn may have effects on mast cell function.
在本研究中，分析了犬肥大细胞瘤细胞系（C2）的脂筏组成。使用无去污剂分离技术，将脂筏与非脂筏质膜很好地分离。为了研究 n-3 和 n-6 多不饱和脂肪酸（PUFA）对与非脂筏质膜相比的脂筏脂肪酸组成的影响，向 C2 中添加以下一种物质：α-亚麻酸、二十碳五烯酸、二十二碳六烯酸、亚油酸或花生四烯酸。用特定的 PUFA 富集培养基会导致这种脂肪酸在脂筏和非脂筏膜中的含量增加。胆固醇和蛋白质的含量不受脂肪酸谱变化的影响。总之，我们的数据提供了强有力的证据，表明 PUFA 调节肥大细胞膜微区的脂质组成和生理特性，这反过来又可能对肥大细胞功能产生影响。
