Grsković Branka, Mrsić Gordan, Vrdoljak Andro, Merkas Sinisa, Andelinović Simun
Forensic Science Centre Ivan Vucetić, General Police Directorate, Ministry of Interior, Ilica 335, 10000 Zagreb, Croatia.
Croat Med J. 2010 Jun;51(3):202-8. doi: 10.3325/cmj.2010.51.202.
To investigate the population genetics of 17 short tandem repeat (STR) loci on the Y chromosome in the population of eastern Croatia.
We carried out a statistical analysis of the data from previously performed genetic analysis collected during routine forensic work by the Forensic Science Centre "Ivan Vucetić". A total of 220 unrelated healthy men from eastern Croatia were selected for the purpose of this study. Genomic DNA was extracted by Chelex from FTA cards. Y-chromosomal STRs were determined using the AmpFISTR Yfiler PCR amplification kit. The haplotype frequencies were determined by direct counting and analyzed using Arlequin 3.1 and analysis of molecular variance calculated with the Y-chromosome haplotype reference database online analysis tool.
A total of 207 haplotypes were recorded, 197 of which were unique (90%). Haplotype diversity was 0.9993, with the most frequent haplotype found in 4 of 220 men (1.8%). Average locus diversity was 0.600, and it ranged from 0.256 for DYS392 to 0.780 for DYS458. Our results were compared with the pattern of Y-chromosome variability in publicly available population samples based on a minimal European haplotype set of 9 STRs and the greatest resemblance was found with samples from the Croatian capital of Zagreb, from Bosnia and Herzegovina, and from Serbia.
This is the first description of Y chromosome haplotyping of the population of eastern Croatia, which may serve as a basis for genetic epidemiology and forensic studies. Further studies are needed for characterization of the genetic structure of the Y-chromosome in the modern Croatian population.
研究克罗地亚东部人群Y染色体上17个短串联重复序列（STR）位点的群体遗传学。
我们对“伊万·武切蒂奇”法医学中心在常规法医工作中收集的先前进行的基因分析数据进行了统计分析。本研究共选取了220名来自克罗地亚东部的无亲缘关系的健康男性。通过Chelex从FTA卡中提取基因组DNA。使用AmpFISTR Yfiler PCR扩增试剂盒测定Y染色体STR。通过直接计数确定单倍型频率，并使用Arlequin 3.1进行分析，并用Y染色体单倍型参考数据库在线分析工具计算分子方差分析。
共记录了207种单倍型，其中197种是独特的（90%）。单倍型多样性为0.9993，最常见的单倍型在220名男性中的4名中出现（1.8%）。平均位点多样性为0.600，范围从DYS392的0.256到DYS458的0.780。我们的结果与基于9个STR的最小欧洲单倍型组的公开可用群体样本中的Y染色体变异模式进行了比较，发现与来自克罗地亚首都萨格勒布、波斯尼亚和黑塞哥维那以及塞尔维亚的样本最为相似。
这是对克罗地亚东部人群Y染色体单倍型的首次描述，可为遗传流行病学和法医研究提供基础。需要进一步研究以表征现代克罗地亚人群中Y染色体的遗传结构。
