Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
J Chromatogr A. 2010 Jul 2;1217(27):4555-60. doi: 10.1016/j.chroma.2010.04.068.
A new approach for preparation of polar-modified reversed-phase liquid chromatography stationary phases was developed by using horizontal polymerization technique on silica surface, which was defined as "polar-copolymerized" approach. Based on this new approach, a representative polar-copolymerized stationary phase composed of mixed n-octadecyl and chloropropyl (C18-C3Cl) was synthesized. The resulting stationary phase named C18HCE was characterized with elemental analysis and solid phase 13C and 29Si NMR, which proved the chemistry of polar-copolymerized stationary phases. Chromatographic evaluation and application of the C18HCE were also investigated. The results of preliminary chromatographic evaluation demonstrated that the C18HCE stationary phase exhibited 100% aqueous mobile phase compatibility, low silanol activity. In addition, the application results demonstrated that the C18HCE had superior separation performance in alkaloids separation at acidic conditions compared to some commercial stationary phases.
开发了一种在硅胶表面上通过水平聚合技术制备极性改性反相液相色谱固定相的新方法，称为“极性共聚”方法。基于这种新方法，合成了一种由混合十八烷基和氯丙基（C18-C3Cl）组成的代表性极性共聚固定相。所得固定相命名为 C18HCE，通过元素分析和固相 13C 和 29Si NMR 进行了表征，证明了极性共聚固定相的化学性质。还研究了 C18HCE 的色谱评价和应用。初步色谱评价结果表明，C18HCE 固定相具有 100%水相流动相兼容性和低硅醇活性。此外，应用结果表明，与一些商业固定相相比，C18HCE 在酸性条件下分离生物碱时具有优异的分离性能。
